Lee Young-Jun, Kim Kui-Jin, Park Kee-Jai, Yoon Bo-Ra, Lim Jeong-Ho, Lee Ok-Hwan
Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea.
Int J Mol Sci. 2013 Jan 11;14(1):1428-42. doi: 10.3390/ijms14011428.
Buckwheat sprouts contain various bioactive compounds including rutin which have a number of biological activities. We have previously shown that buckwheat sprouts (TBWE) treated with methyl jasmonate (MeJA) significantly increased the amount of phenolics and the antioxidant activity. The aim of this study was to demonstrate the effect of TBWE on anti-adipogenesis and pro-oxidant enzyme in 3T3-L1 adipocytes. We also evaluated the anti-oxidative activity of TBWE in adipocytes by using the nitroblue tetrazolium assay. Our data showed that TBWE markedly inhibited adipocyte differentiation and ROS production in 3T3-L1 cells compared with control groups. Moreover, TBWE has strongly shown the inhibition of adipogenic transcription factor as well as pro-oxidant enzymes. Together, we demonstrate that the MeJA treatment significantly increased the amount of phenolic compound, resulting in the suppression of adipogenesis and ROS production in the 3T3-L1 cells. These findings indicate that TBWE has the potential for anti-adipogenesis activity with anti-oxidative properties.
荞麦芽含有多种生物活性化合物，包括具有多种生物活性的芦丁。我们之前已经表明，用茉莉酸甲酯（MeJA）处理的荞麦芽（TBWE）显著增加了酚类物质的含量和抗氧化活性。本研究的目的是证明TBWE对3T3-L1脂肪细胞抗脂肪生成和促氧化酶的影响。我们还通过使用硝基蓝四唑试验评估了TBWE在脂肪细胞中的抗氧化活性。我们的数据表明，与对照组相比，TBWE显著抑制了3T3-L1细胞中的脂肪细胞分化和活性氧（ROS）产生。此外，TBWE强烈显示出对脂肪生成转录因子以及促氧化酶的抑制作用。总之，我们证明MeJA处理显著增加了酚类化合物的含量，导致3T3-L1细胞中脂肪生成和ROS产生受到抑制。这些发现表明，TBWE具有抗脂肪生成活性和抗氧化特性的潜力。
