Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Clin Chem. 2013 Feb;59(2):392-400. doi: 10.1373/clinchem.2012.193813.
Public sharing of scientific data has assumed greater importance in the omics era. Transparency is necessary for confirmation and validation, and multiple examiners aid in extracting maximal value from large data sets. Accordingly, database submission and provision of the Minimum Information About a Microarray Experiment (MIAME)(3) are required by most journals as a prerequisite for review or acceptance.
In this study, the level of data submission and MIAME compliance was reviewed for 127 articles that included microarray-based microRNA (miRNA) profiling and were published from July 2011 through April 2012 in the journals that published the largest number of such articles--PLOS ONE, the Journal of Biological Chemistry, Blood, and Oncogene--along with articles from 9 other journals, including Clinical Chemistry, that published smaller numbers of array-based articles.
Overall, data submission was reported at publication for <40% of all articles, and almost 75% of articles were MIAME noncompliant. On average, articles that included full data submission scored significantly higher on a quality metric than articles with limited or no data submission, and studies with adequate description of methods disproportionately included larger numbers of experimental repeats. Finally, for several articles that were not MIAME compliant, data reanalysis revealed less than complete support for the published conclusions, in 1 case leading to retraction.
These findings buttress the hypothesis that reluctance to share data is associated with low study quality and suggest that most miRNA array investigations are underpowered and/or potentially compromised by a lack of appropriate reporting and data submission.
在组学时代，公共科学数据共享的重要性日益增加。透明性是确认和验证的必要条件，多位审查员有助于从大数据集中提取最大价值。因此，大多数期刊都要求数据库提交和提供微阵列实验的最低信息（MIAME）（3）作为审查或接受的前提条件。
在这项研究中，我们审查了 127 篇基于微阵列的 microRNA (miRNA) 分析文章的数据提交和 MIAME 合规性，这些文章发表于 2011 年 7 月至 2012 年 4 月期间，发表这些文章的期刊包括发表此类文章数量最多的 PLoS ONE、《生物化学杂志》、《血液》和《癌基因》，以及其他 9 种期刊，包括发表较少基于阵列文章的《临床化学》。
总体而言，所有文章中只有不到 40%的文章在发表时报告了数据提交情况，近 75%的文章不符合 MIAME 标准。平均而言，包括完整数据提交的文章在质量指标上的得分明显高于数据提交有限或没有数据提交的文章，并且对方法进行充分描述的研究实验重复次数也不成比例地增加。最后，对于一些不符合 MIAME 标准的文章，数据重新分析显示，发表的结论并不完全支持，在 1 个案例中导致撤回。
这些发现支持了这样一种假设，即不愿意共享数据与研究质量低有关，并表明大多数 miRNA 阵列研究的力度不足，并且/或者由于缺乏适当的报告和数据提交，存在潜在的风险。
