Center for Advanced Brain Imaging, Nathan Kline Institute, 140 Old Orangeburg Road, Orangeburg, NY 10962, USA.
J Neurosci Methods. 2013 Apr 15;214(2):144-8. doi: 10.1016/j.jneumeth.2013.01.019. Epub 2013 Jan 31.
Although many resting state fMRI human studies have been published, the number of such rodent studies is considerably less. The reason for this is the severe technical challenge of high magnetic field small rodent imaging. Local magnetic field susceptibility changes at air tissue boundaries cause image distortion and signal losses. The current study reports measures of functional connectivity in mice using only isoflurane for the anesthetic. Because all anesthetic agents will alter cerebral blood flow and cerebral metabolism, the impact these changes have on neuronal connectivity has yet to be fully understood, however this work reports for the first time that reliable functional connectivity measures in mouse brain can be obtained with isoflurane.
尽管已经发表了许多基于静息态 fMRI 的人类研究，但类似的啮齿动物研究数量要少得多。造成这种情况的原因是高磁场小型啮齿动物成像的技术挑战非常大。空气组织边界处的局部磁场磁化率变化会导致图像变形和信号丢失。本研究报告了仅使用异氟烷作为麻醉剂的小鼠功能连接的测量结果。由于所有麻醉剂都会改变脑血流和脑代谢，因此这些变化对神经元连接的影响尚未完全了解，但这项工作首次报告了使用异氟烷可以在小鼠大脑中获得可靠的功能连接测量结果。
