School of Preclinic, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand.
Department of Pharmacology, Phramongkutklao College of Medicine, Bangkok, 10400, Thailand.
Adv Exp Med Biol. 2017;975 Pt 1:295-305. doi: 10.1007/978-94-024-1079-2_26.
This study tests the hypothesis that perinatal taurine supplementation prevents diabetes mellitus and hypertension in adult offspring of maternal diabetic rats. Female Wistar rats were fed normal rat chow and tap water with (Diabetes group) or without diabetic induction by intraperitoneal streptozotocin injection (Control group) before pregnancy. Then, they were supplemented with 3% taurine in water (Control+T and Diabetes+T groups) or water alone from conception to weaning. After weaning, both male and female offspring were fed normal rat chow and tap water throughout the study. Blood chemistry and cardiovascular parameters were studied in 16-week old rats. Body, heart, and kidney weights were not significantly different among the eight groups. Further, lipid profiles except triglyceride were not significantly different among male and female groups, while male Diabetes displayed increased fasting blood glucose, decreased plasma insulin, and increased plasma triglyceride compared to other groups. Compared to Control, mean arterial pressures significantly increased and baroreflex control of heart rate decreased in both male and female Diabetes, while heart rates significantly decreased in male but increased in female Diabetes group. Although perinatal taurine supplementation did not affect any measured parameters in Control groups, it abolished the adverse effects of maternal diabetes on fasting blood glucose, plasma insulin, lipid profiles, mean arterial pressure, heart rate, and baroreflex sensitivity in adult male and female offspring. The present study indicates that maternal diabetes mellitus induces metabolic and cardiovascular defects more in male than female adult offspring, and these adverse effects can be prevented by perinatal taurine supplementation.
这项研究检验了这样一个假设，即围产期牛磺酸补充可以预防母鼠糖尿病成年后代的糖尿病和高血压。在怀孕前，雌性 Wistar 大鼠喂食普通大鼠饲料和自来水，或者通过腹腔注射链脲佐菌素进行糖尿病诱导（糖尿病组）（对照组）。然后，它们从受孕到断奶期间用 3%牛磺酸水（对照组+T 和糖尿病+T 组）或单独用水补充。断奶后，雄性和雌性后代在整个研究期间均喂食普通大鼠饲料和自来水。在 16 周龄大鼠中研究了血液化学和心血管参数。八组之间的体重、心脏和肾脏重量没有显著差异。此外，除了甘油三酯之外，雄性和雌性各组的血脂谱没有显著差异，而与其他组相比，雄性糖尿病的空腹血糖升高，血浆胰岛素降低，血浆甘油三酯升高。与对照组相比，雄性和雌性糖尿病组的平均动脉压显著升高，心率的压力反射控制降低，而雄性糖尿病组的心率显著降低，而雌性糖尿病组的心率升高。尽管围产期牛磺酸补充并没有影响对照组中任何测量参数，但它消除了母鼠糖尿病对成年雄性和雌性后代空腹血糖、血浆胰岛素、血脂谱、平均动脉压、心率和压力反射敏感性的不良影响。本研究表明，母鼠糖尿病在成年雄性后代中引起的代谢和心血管缺陷比雌性更多，而这些不良影响可以通过围产期牛磺酸补充来预防。
