Health Strategic Research Center, Neyshabur Medical University of Sciences, Neyshabur, Iran.
Toxicol Mech Methods. 2013 Jul;23(6):432-6. doi: 10.3109/15376516.2013.777136. Epub 2013 Mar 13.
Despite the wide spread of lead environmental pollution, the effect of this heavy metal on respiratory disease was not shown yet. In respect to increased oxidative stress is an important mechanism in the pathogenesis of respiratory disease, the present study was designed to examine the association between lead toxicity and lung disease via measuring oxidative stress biomarkers in bronchoalveolar lavage fluid (BALF) and lung tissue of rat. For this aim, 32 rats were divided into the following groups of eight animals each: control, three lead tested (received lead acetate in the drinking water for a period of 14 d at concentrations of 250, 500 and 1000 ppm) groups. At the end of the 2 week period, malondialdehyde (MDA), nitric oxide (NO) and reduced glutathione (GSH) contents were measured to assess free radical activity in the BALF and lung tissue. Superoxide dismutase (SOD) was also determined. A significant dose-dependent increase in the BALF supernatant and lung homogenate levels of MDA and NO with decrease GSH level and SOD activity were observed in the lead-treated groups compared with the control group (p < 0.05). Thus, lead acetate may be contributed to respiratory disorders via increased oxidative stress.
尽管铅的环境污染广泛存在，但这种重金属对呼吸道疾病的影响尚未显现。鉴于氧化应激增加是呼吸道疾病发病机制中的一个重要机制，本研究旨在通过测量支气管肺泡灌洗液（BALF）和大鼠肺组织中的氧化应激生物标志物来研究铅毒性与肺部疾病之间的关联。为此，将 32 只大鼠分为以下 8 组，每组 8 只：对照组、3 个铅暴露组（在饮用水中分别以 250、500 和 1000ppm 的浓度连续 14 天摄入醋酸铅）。在 2 周的研究结束时，测量丙二醛（MDA）、一氧化氮（NO）和还原型谷胱甘肽（GSH）的含量，以评估 BALF 和肺组织中的自由基活性。还测定了超氧化物歧化酶（SOD）的含量。与对照组相比，铅处理组 BALF 上清液和肺组织匀浆中的 MDA 和 NO 水平呈显著的剂量依赖性增加，而 GSH 水平和 SOD 活性则降低（p<0.05）。因此，醋酸铅可能通过增加氧化应激导致呼吸道疾病。
