Portelli Lucas A, Schomay Theodore E, Barnes Frank S
Department of Electrical, Computer and Energy Engineering, University of Colorado at Boulder, Colorado, USA.
Bioelectromagnetics. 2013 Jul;34(5):337-48. doi: 10.1002/bem.21787. Epub 2013 Mar 1.
This report shows that the background magnetic field in biological incubators can vary by orders of magnitude within and between incubators. These variations can be observed within the same incubator in locations that are centimeters apart from each other as well as between incubators that are identical and located in the same laboratory. Additionally, the values measured were frequently outside the range of magnitudes found naturally on the Earth's surface or ordinary habitation spaces. Exposure to such altered magnetic field environments has been experimentally shown to be sufficient to cause numerous effects in cell cultures. Examples of the effects reported span from differential generation of free radicals and heat shock proteins to differences in cellular proliferation, differentiation, and death. Although the effects are not well established and the molecular mechanism of action is currently under debate, these observations alone support the notion that the inhomogeneity of the background magnetic field in incubators is a potential confounding source of the variability and reproducibility for studies performed on cell cultures. In this regard, it is recommended that special measures be adopted to control the background magnetic fields in incubators when investigating the biological effects of exposure to magnetic fields of comparable characteristics as the ones measured in this study, or when studying small biological effects in general.
本报告表明，生物培养箱内的背景磁场在不同培养箱之间以及同一培养箱内部可能会有几个数量级的变化。在同一培养箱内，相距仅几厘米的位置之间，以及位于同一实验室的相同培养箱之间，都能观察到这种变化。此外，所测得的值常常超出地球表面或普通居住空间自然存在的磁场强度范围。实验表明，暴露于这种改变了的磁场环境足以在细胞培养中引发多种效应。报告的效应实例包括自由基和热休克蛋白的差异产生，以及细胞增殖、分化和死亡的差异。尽管这些效应尚未完全明确，其分子作用机制目前也存在争议，但仅这些观察结果就支持了这样一种观点，即培养箱内背景磁场的不均匀性是细胞培养研究中变异性和可重复性的一个潜在混杂来源。在这方面，建议在研究与本研究中所测磁场具有可比特征的磁场暴露的生物学效应时，或者在一般研究微小生物学效应时，采取特殊措施来控制培养箱内的背景磁场。
