Spiritual strategies may provide an effective way of coping with disease. This study sought to investigate the validity and reliability of the Persian version of the spiritual coping strategies (SCS) scale among Iranian hemodialysis patients. A convenience sample of 204 hemodialysis patients was recruited to participate in the study. A forward-backward translation method was used to produce the Persian version of the scale. Internal consistency was assessed by Cronbach's alpha and item-total score correlation. Two-week test-retest reliability was also assessed. The convergent and divergent validity of the scale was evaluated using the Duke University Religion Index and a visual analogue scale for health status. Exploratory and confirmatory factor analyses were used to assess the factor structure. Participants consisted of 113 males and 91 females (mean age 57.2 [SD = 9.7]). Cronbach's alpha was acceptable (0.87). We found two underlying factors similar to the original scale. The correlations between the study scales confirmed the convergent and divergent validity of the SCS. Confirmatory factor analysis showed a good fit to the data (GFI = 0.923, CFI = 0.948 and RMSEA = 0.068). The Persian version of the SCS has sound psychometric properties in Iranian hemodialysis patients. Future research should consider applying the scale to populations with other religious/cultural backgrounds.