Singh Satnam, C P S Yadav, Noolvi Malleshappa N
Department of Pharmaceutical Sciences, ASBASJSM College of Pharmacy, Bela (Ropar)-140111, Punjab, India.
Asian Pac J Trop Biomed. 2012 Jun;2(6):433-7. doi: 10.1016/S2221-1691(12)60071-9.
To explore the immunomodulatory properties of 80% ethanol extract and butanol fraction of Gentiana olivieri (G. olivieri) Griseb on Balb/C mice.
The study was performed with basic models of immunomodulation such as the humoral antibody response (hemoglutination antibody titres), cell mediated immune response (delayed type hypersensitivity and in vivo carbon clearance or phagocytosis). Ethanol (80%) extract of flowering aerial parts of G. olivieri and its butanol fraction were administered p.o. (orally) to the mice. Levamisole, 2.5 mg/kg was used as standard drug.
There was a potentiation of immune response to sheep red blood cells by cellular and humoral mediated mechanisms comparable to levamisole (2.5 mg/kg) by both 80% ethanol extract and the butanol fraction at doses of 50-200 mg/kg in male Balb/C mice. Both significantly (P<0.01) potentiated the humoral immune response in cyclophosphamide (250 mg/kg) immunosupressed mice at 100 and 200 mg/kg of each extract and fraction as compared to control. The potentiation of delayed type hypersensitivity response was statistically significant (P<0.01) at 200 mg/kg of ethanol extract and 100, 200 mg/kg of butanol fraction as compared to control. The phagocytosis was significant at 200 mg/kg with butanol fraction of G. olivieri.
The results reveal the immunostimulant effects of plant G. olivieri in mice by acting through cellular and humoral immunity in experimental models of immunity in mice. Butanol fraction is the most effective at a dose level of 200 mg/kg.
探讨川西獐牙菜80%乙醇提取物及正丁醇部位对Balb/C小鼠的免疫调节特性。
采用体液抗体反应（血凝抗体效价）、细胞介导免疫反应（迟发型超敏反应及体内碳粒廓清或吞噬作用）等免疫调节基础模型进行研究。将川西獐牙菜开花地上部分的乙醇（80%）提取物及其正丁醇部位经口（口服）给予小鼠。以2.5mg/kg左旋咪唑作为标准药物。
在雄性Balb/C小鼠中，80%乙醇提取物和正丁醇部位在50 - 200mg/kg剂量下，通过细胞和体液介导机制增强了对绵羊红细胞的免疫反应，与2.5mg/kg左旋咪唑相当。与对照组相比，各提取物和部位在100mg/kg和200mg/kg剂量下均显著（P<0.01）增强了环磷酰胺（250mg/kg）免疫抑制小鼠的体液免疫反应。与对照组相比，乙醇提取物200mg/kg剂量及正丁醇部位100mg/kg、200mg/kg剂量下迟发型超敏反应增强具有统计学意义（P<0.01）。川西獐牙菜正丁醇部位在200mg/kg剂量下吞噬作用显著。
结果揭示了川西獐牙菜在小鼠免疫实验模型中通过细胞免疫和体液免疫发挥免疫刺激作用。正丁醇部位在200mg/kg剂量水平下最有效。
