Center for Applied Molecular Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.
Sci Rep. 2013;3:1449. doi: 10.1038/srep01449.
To investigate the transition from non-cancerous to metastatic from a physical sciences perspective, the Physical Sciences-Oncology Centers (PS-OC) Network performed molecular and biophysical comparative studies of the non-tumorigenic MCF-10A and metastatic MDA-MB-231 breast epithelial cell lines, commonly used as models of cancer metastasis. Experiments were performed in 20 laboratories from 12 PS-OCs. Each laboratory was supplied with identical aliquots and common reagents and culture protocols. Analyses of these measurements revealed dramatic differences in their mechanics, migration, adhesion, oxygen response, and proteomic profiles. Model-based multi-omics approaches identified key differences between these cells' regulatory networks involved in morphology and survival. These results provide a multifaceted description of cellular parameters of two widely used cell lines and demonstrate the value of the PS-OC Network approach for integration of diverse experimental observations to elucidate the phenotypes associated with cancer metastasis.
为了从物理科学的角度研究从非癌性向转移性的转变，物理科学-肿瘤学中心（PS-OC）网络对非致瘤性 MCF-10A 和转移性 MDA-MB-231 乳腺上皮细胞系进行了分子和生物物理比较研究，这些细胞系通常被用作癌症转移的模型。实验在 12 个 PS-OC 的 20 个实验室中进行。每个实验室都提供了相同的等分试样和常用试剂和培养方案。对这些测量结果的分析显示，它们的力学、迁移、粘附、氧反应和蛋白质组学特征存在显著差异。基于模型的多组学方法确定了这两种细胞形态和存活相关的调节网络之间的关键差异。这些结果提供了两种广泛使用的细胞系的细胞参数的多方面描述，并证明了 PS-OC 网络方法的价值，该方法可整合各种实验观察结果，以阐明与癌症转移相关的表型。
