Laboratoire de Bioingénierie et Biomécanique Ostéoarticulaire, U.M.R.-C.N.R.S. 7052, 10, avenue de Verdun, 75010, Paris, France.
J Mater Sci Mater Med. 2010 Feb;21(2):707-15. doi: 10.1007/s10856-009-3928-5. Epub 2009 Nov 10.
The present study assessed in vivo new bone formation around titanium alloy implants chemically grafted with macromolecules bearing ionic sulfonate and/or carboxylate groups. Unmodified and grafted Ti-6Al-4V exhibiting either 100% carboxylate, or 100% sulfonate, or both carboxylate and sulfonate groups in the percent of 50/50 and 80/20 were bilaterally implanted into rabbit femoral condyle. Neither toxicity nor inflammation were observed for all implants tested. After 4 weeks, peri-implant new bone formation varied as a function of the chemical composition of the titanium surfaces. The percent bone-implant contact (BIC) was the lowest (13.4 +/- 6.3%) for the implants modified with grafted carboxylate only. The value of BIC on the implants with 20% sulfonate (24.6 +/- 5.2%) was significantly (P < 0.05) lower than that observed on 100% sulfonate (38.2 +/- 13.2%) surfaces. After both 4 and 12 weeks post-implantation, the BIC value for implants with more than 50% sulfonate was similar to that obtained with the unmodified Ti-6Al-4V. The grafted titanium alloy exhibiting either 100% sulfonate or carboxylate and sulfonate (50% each) groups promoted bone formation. Such materials are of clinical interest because, they do not promote bacteria adhesion but, they support new bone formation, a condition which can lead to osseointegration of bone implants while preventing peri-implant infections.
本研究评估了经化学接枝带有离子磺酸根和/或羧酸盐基团的大分子后，钛合金植入物体内新骨形成情况。未修饰和接枝的 Ti-6Al-4V 分别具有 100%羧酸盐、100%磺酸根或 50/50 和 80/20 的羧酸盐和磺酸根，双侧植入兔股骨髁。所有测试的植入物均未观察到毒性或炎症。4 周后，骨整合的新骨形成随钛表面化学成分的变化而变化。仅用接枝羧酸盐修饰的植入物的骨-植入物接触率（BIC）最低（13.4%±6.3%）。20%磺酸根（24.6%±5.2%）的植入物的 BIC 值明显低于 100%磺酸根（38.2%±13.2%）表面。植入后 4 周和 12 周后，BIC 值大于 50%磺酸根的植入物与未修饰的 Ti-6Al-4V 相似。具有 100%磺酸根或磺酸根和羧酸盐（各 50%）基团的接枝钛合金促进骨形成。这些材料具有临床意义，因为它们不促进细菌黏附，但支持新骨形成，这可以导致骨植入物的骨整合，同时防止植入物周围感染。
