Department of Chemistry, Jinan University, Guangzhou 510632, P R China.
ACS Appl Mater Interfaces. 2013 Jun 12;5(11):4760-8. doi: 10.1021/am4000485. Epub 2013 May 23.
Reduced graphene oxide (RGO) and silver nanoparticle (AgNP) hybrids (RGO-AgNP) were prepared by a facile one-pot method using Poly (N-vinyl-2-pyrrolidone) as reductant and stabilizer. Folic acid (FA) molecules were attached to the RGO-AgNP by physisorption for targeting specific cancer cells with folate receptors (FRs) and using as Raman reporter molecules. The internalization of the FA loaded RGO-AgNP (RGO-AgNP-FA) inside the FRs-positive cancer cell was confirmed by confocal laser scanning and transmission electron microscopy. The Raman signals of the FA in live cancer cells were detected by confocal Raman spectroscope at 514 nm excitation, indicating that the RGO-AgNP-FA material has great potential as a Raman probe for cancer diagnosis in vitro.
通过简便的一锅法制备了还原氧化石墨烯（RGO）和银纳米粒子（AgNP）的复合物（RGO-AgNP），其中聚（N-乙烯基-2-吡咯烷酮）作为还原剂和稳定剂。通过物理吸附将叶酸（FA）分子连接到 RGO-AgNP 上，以靶向具有叶酸受体（FRs）的特定癌细胞，并用作拉曼报告分子。通过共焦激光扫描和透射电子显微镜证实了 FA 负载的 RGO-AgNP（RGO-AgNP-FA）进入 FRs 阳性癌细胞内的内化。在 514nm 激发下，通过共聚焦 Raman 光谱仪检测到活癌细胞中 FA 的 Raman 信号，表明 RGO-AgNP-FA 材料具有作为体外癌症诊断的拉曼探针的巨大潜力。
