Consiglio Nazionale delle Ricerche, Istituto per la Sintesi Organica e la Fotoreattività, via Gobetti, 101, 40129 Bologna, Italy.
Nat Mater. 2013 Jul;12(7):672-80. doi: 10.1038/nmat3630. Epub 2013 May 5.
Real-time stimulation and recording of neural cell bioelectrical activity could provide an unprecedented insight in understanding the functions of the nervous system, and it is crucial for developing advanced in vitro drug screening approaches. Among organic materials, suitable candidates for cell interfacing can be found that combine long-term biocompatibility and mechanical flexibility. Here, we report on transparent organic cell stimulating and sensing transistors (O-CSTs), which provide bidirectional stimulation and recording of primary neurons. We demonstrate that the device enables depolarization and hyperpolarization of the primary neuron membrane potential. The transparency of the device also allows the optical imaging of the modulation of the neuron bioelectrical activity. The maximal amplitude-to-noise ratio of the extracellular recording achieved by the O-CST device exceeds that of a microelectrode array system on the same neuronal preparation by a factor of 16. Our organic cell stimulating and sensing device paves the way to a new generation of devices for stimulation, manipulation and recording of cell bioelectrical activity in vitro and in vivo.
实时刺激和记录神经细胞生物电活动可以提供对神经系统功能的前所未有的深入了解，这对于开发先进的体外药物筛选方法至关重要。在有机材料中，可以找到适合细胞界面的候选材料，它们结合了长期的生物相容性和机械灵活性。在这里，我们报告了透明的有机细胞刺激和传感晶体管 (O-CST)，它可以双向刺激和记录原代神经元。我们证明了该器件能够使原代神经元膜电位去极化和超极化。该器件的透明性还允许对神经元生物电活动的调制进行光学成像。O-CST 器件实现的细胞外记录的最大幅度噪声比是同一神经元制备的微电极阵列系统的 16 倍。我们的有机细胞刺激和传感器件为刺激、操作和记录体外和体内细胞生物电活动开辟了新的途径。
