Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia, USA.
Br J Ophthalmol. 2013 Jul;97(7):929-33. doi: 10.1136/bjophthalmol-2013-303343. Epub 2013 May 4.
To evaluate the use of in vivo imaging of rabbit model of choroidal melanoma using high-frequency contrast-enhanced ultrasound (HF-CE-US) with two-dimensional (2D) or three-dimensional (3D) modes and to correlate the sonographic findings with histopathologic characteristics.
Five New Zealand white rabbits, which were immunosuppressed with daily cyclosporin A (CsA), were inoculated into their right eyes with aliquots of 1.5×10(6)/50 μl of 92.1 human uveal melanoma cells cultured in RPMI. At week 4, the tumour-bearing eyes were imaged using high-frequency ultrasound (HF-US) with microbubble contrast agent to determine the 2D tumour size and relative blood volume and by 3D mode to determine tumour volume. Histologic tumour burden was quantified in enucleated eyes by ImageJ software, and mean vascular density (MVD) was determined by counting vascular channels in periodic acid Schiff (PAS) without haematoxylin sections.
Using HF-CE-US, melanomas were visualised as relatively hyperechoic regions in the images. The correlation coefficients of sonographic size and volume compared with histologic area were 0.72 and 0.70, respectively. The sonographic tumour relative blood volume correlated with the histologic tumour vascularity (r(2)=0.92, p=0.04).
There is a positive correlation between in vivo sonographic tumour volume/size and histologic tumour size in our rabbit choroidal melanoma model. HF-CE-US corresponds to MVD and blood volume.
利用高频超声（HF-US）联合二维（2D）或三维（3D）对比增强模式对兔眼脉络膜黑色素瘤模型进行体内成像，并将超声结果与组织病理学特征相关联。
5 只新西兰白兔，每天给予环孢素 A（CsA）免疫抑制，通过右眼球接种 1.5×10(6)/50 μl 的 92.1 人葡萄膜黑色素瘤细胞，在 RPMI 中培养。在第 4 周，使用高频超声（HF-US）联合微泡造影剂对荷瘤眼进行成像，以确定 2D 肿瘤大小和相对血容量，并通过 3D 模式确定肿瘤体积。通过 ImageJ 软件对眼球进行定量分析，计算组织学肿瘤负担，通过在无苏木精染色的过碘酸雪夫（PAS）切片中计数血管通道，确定平均血管密度（MVD）。
使用 HF-CE-US，黑色素瘤在图像中呈现相对高回声区域。超声大小和体积与组织学面积的相关系数分别为 0.72 和 0.70。超声肿瘤相对血容量与组织学肿瘤血管生成（r(2)=0.92，p=0.04）相关。
在我们的兔眼脉络膜黑色素瘤模型中，体内超声肿瘤体积/大小与组织学肿瘤大小呈正相关。HF-CE-US 与 MVD 和血容量相对应。
