Gegić Merima, Numanović Fatima, Delibegović Zineta, Tihić Nijaz, Nurkić Mahmut, Hukić Mirsada
Tuzla University Clinic Centre, Clinic for Laboratory Diagnosis, Department of Microbiology, Tuzla, Bosnia and Herzegovina.
Coll Antropol. 2013 Mar;37(1):157-63.
Candidiasis is defined as an infection or disease caused by a fungus of the genus Candida. Rate of disseminated candidiasis increases with the growth of the number of immunocompromised patients. In the the last few decades the incidence of disseminated candidiasis is in growth as well as the mortality rate. The aim of this survey is to show the importance of serological tests implementation in disseminated candidiasis diagnose. This is a prospective study involving 60 patients with malign diseases with and without clinical signs of disseminated candidiasis and 30 healthy people who represent the control group. Apart from hemoculture, detection of circulating mannan antigen and adequate antibodies of Candida species applying comercial ELISA test was determined in each patient. This survey deals with relevant factors causing disseminated candidiasis. This survey showed that the group of patients with clinical signs of disseminated candidiasis had more patients with positive hemoculture to Candida species, then the group of patients without clinical signs of disseminated candidiasis. The number of patients being examined and positive to antigens and antibodies was higher (p < 0.01) in the group of patients with clinical signs of disseminated candidiasis (7/30; 23.3%), then in the group of patients without clinical signs of disseminated candidiasis (0/30; 0%): Average value of titra antigen was statistically higher (p < 0.001) in patients with Candida spp. positive hemocultures rather then in patients with Candida spp. negative hemocultures. In the group of patients with clinical signs of disseminated candidiasis 6/30 (20%) of patients had Candida spp.positive hemocultures while in the group of patients without clinical signs of disseminated candidiasis 1/30 (3.3%) of patients had Candida spp. positive hemocultures, which was considerably higher (p < 0.05). Correlation of results of hemoculture and mannan antigens and antibodies in patients with disseminated candidiasis were statistically significant, while correlation of results of hemoculture and antibodies was insignificant. Because of low sensitivity of hemoculture and time needed for isolation of Candida spp., introducing serological tests in regular procedures would speed disseminated candidiasis diagnose.
念珠菌病被定义为由念珠菌属真菌引起的感染或疾病。随着免疫功能低下患者数量的增加，播散性念珠菌病的发病率也在上升。在过去几十年中，播散性念珠菌病的发病率和死亡率都在上升。本调查的目的是展示血清学检测在播散性念珠菌病诊断中的重要性。这是一项前瞻性研究，涉及60例患有恶性疾病且有或无播散性念珠菌病临床症状的患者以及30名作为对照组的健康人。除了血培养外，还对每位患者进行了应用商业ELISA检测法检测循环甘露聚糖抗原和念珠菌属的相应抗体。本调查探讨了导致播散性念珠菌病的相关因素。该调查表明，有播散性念珠菌病临床症状的患者组中血培养念珠菌属阳性的患者比无播散性念珠菌病临床症状的患者组更多。有播散性念珠菌病临床症状的患者组中接受检测且抗原和抗体呈阳性的患者数量（7/30；23.3%）高于无播散性念珠菌病临床症状的患者组（0/30；0%），差异有统计学意义（p < 0.01）：念珠菌属血培养阳性患者的抗原滴度平均值在统计学上高于念珠菌属血培养阴性患者（p < 0.001）。有播散性念珠菌病临床症状的患者组中有6/30（20%）的患者血培养念珠菌属阳性，而无播散性念珠菌病临床症状的患者组中有1/30（3.3%）的患者血培养念珠菌属阳性，差异有统计学意义（p < 0.05）。播散性念珠菌病患者血培养结果与甘露聚糖抗原及抗体的相关性具有统计学意义，而血培养结果与抗体的相关性无统计学意义。由于血培养敏感性低且分离念珠菌属需要时间，在常规程序中引入血清学检测将加快播散性念珠菌病的诊断。
