Wu Yue-Hong, Yan Jing, Fang Chen, Huo Ying-Yi, Ma Wei-Lin, Zhang De-Min, Wang Chun-Sheng, Xu Xue-Wei
Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
Int J Syst Evol Microbiol. 2018 May;68(5):1713-1718. doi: 10.1099/ijsem.0.002734. Epub 2018 Apr 3.
A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated LA399, was isolated from deep-sea sediment collected from the Pacific Ocean. Cells of strain LA399 grew in the medium containing 0-10.0 % of NaCl (w/v; optimum 3.0-5.0 %), pH 6.5-8.0 (optimum 7.0) and 20-40 °C (optimum 37 °C). Aesculin, gelatin, starch and Tween 80 were hydrolysed. Strain LA399 was closely related to Gracilimonas halophila WDS2C40 (97.0 % sequence similarity), Gracilimonas mengyeensis YIM J14 (96.4 %), Gracilimonas rosea CL-KR2 (96.4 %) and Gracilimonas tropica DSM 19535 (96.0 %), and exhibited equal or less than 96.0 % sequence similarity to other type strains of species with validly published names. Phylogenetic analyses revealed that strain LA399 clustered with the clade comprising the Gracilimonas species and formed an independent lineage. Strain LA399 contained menaquinone 7 as the sole isoprenoid quinone and iso-C15 : 0, anteiso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and summed feature 9 (iso-C17 : 1ω9c/10-methyl C16 : 0) as the predominant cellular fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and three unidentified glycolipids. The DNA G+C content was 45.3 mol%. According to the phylogenetic, chemotaxonomic and phenotypic data, it represents a novel species of the genus Gracilimonas, for which the name Gracilimonas amylolytica is proposed. The type strain is LA399 (=CGMCC 1.16248=KCTC 52885).
从太平洋采集的深海沉积物中分离出一株革兰氏阴性、需氧、无运动性的杆状细菌，命名为LA399。LA399菌株能在含0-10.0%（w/v；最适为3.0-5.0%）NaCl、pH 6.5-8.0（最适7.0）和20-40℃（最适37℃）的培养基中生长。七叶苷、明胶、淀粉和吐温80能被水解。LA399菌株与嗜盐纤细单胞菌WDS2C40（序列相似性97.0%）、勐野江纤细单胞菌YIM J14（96.4%）、玫瑰色纤细单胞菌CL-KR2（96.4%）和热带纤细单胞菌DSM 19535（96.0%）密切相关，与其他有效发表名称的种的模式菌株的序列相似性均等于或低于96.0%。系统发育分析表明，LA399菌株与包含纤细单胞菌种的进化枝聚类，并形成一个独立的分支。LA399菌株含有甲基萘醌7作为唯一的类异戊二烯醌，主要细胞脂肪酸为异-C15:0、anteiso-C15:0、特征性总和3（C16:1ω7c/C16:1ω6c）和特征性总和9（异-C17:1ω9c/10-甲基C16:0）。主要极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的磷脂和三种未鉴定的糖脂。DNA G+C含量为45.3 mol%。根据系统发育、化学分类和表型数据，它代表了纤细单胞菌属的一个新种，为此提出名为解淀粉纤细单胞菌的名称。模式菌株为LA399（=CGMCC 1.16248=KCTC 52885）。
