Gracilimonas amylolytica sp. nov., isolated from deep-sea sediment.

A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated LA399, was isolated from deep-sea sediment collected from the Pacific Ocean. Cells of strain LA399 grew in the medium containing 0-10.0 % of NaCl (w/v; optimum 3.0-5.0 %), pH 6.5-8.0 (optimum 7.0) and 20-40 °C (optimum 37 °C). Aesculin, gelatin, starch and Tween 80 were hydrolysed. Strain LA399 was closely related to Gracilimonas halophila WDS2C40 (97.0 % sequence similarity), Gracilimonas mengyeensis YIM J14 (96.4 %), Gracilimonas rosea CL-KR2 (96.4 %) and Gracilimonas tropica DSM 19535 (96.0 %), and exhibited equal or less than 96.0 % sequence similarity to other type strains of species with validly published names. Phylogenetic analyses revealed that strain LA399 clustered with the clade comprising the Gracilimonas species and formed an independent lineage. Strain LA399 contained menaquinone 7 as the sole isoprenoid quinone and iso-C15 : 0, anteiso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and summed feature 9 (iso-C17 : 1ω9c/10-methyl C16 : 0) as the predominant cellular fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and three unidentified glycolipids. The DNA G+C content was 45.3 mol%. According to the phylogenetic, chemotaxonomic and phenotypic data, it represents a novel species of the genus Gracilimonas, for which the name Gracilimonas amylolytica is proposed. The type strain is LA399 (=CGMCC 1.16248=KCTC 52885).