Department of Genetic Medicine and Development, University of Geneva Medical School, Geneva, Switzerland.
Nucleic Acids Res. 2013 Jul;41(Web Server issue):W165-8. doi: 10.1093/nar/gkt430. Epub 2013 May 28.
MicroRNAs (miRNAs) posttranscriptionally repress the expression of protein-coding genes. Based on the partial complementarity between miRNA and messenger RNA pairs with a mandatory so-called 'seed' sequence, many thousands of potential targets can be identified. Our open-source software library, miRmap, ranks these potential targets with a biologically meaningful criterion, the repression strength. MiRmap combines thermodynamic, evolutionary, probabilistic and sequence-based features, which cover features from TargetScan, PITA, PACMIT and miRanda. Our miRmap web application offers a user-friendly and feature-rich resource for browsing precomputed miRNA target predictions for model organisms, as well as for predicting and ranking targets for user-submitted sequences. MiRmap web integrates sorting, filtering and exporting of results from multiple queries, as well as providing programmatic access, and is available at http://mirmap.ezlab.org.
MicroRNAs (miRNAs) 在后转录水平上抑制蛋白质编码基因的表达。基于 miRNA 和信使 RNA 对之间的部分互补性，具有强制性的所谓“种子”序列，可以识别出成千上万的潜在靶标。我们的开源软件库 miRmap 用一种具有生物学意义的标准，即抑制强度，对这些潜在的靶标进行排名。MiRmap 结合了热力学、进化、概率和基于序列的特征，涵盖了来自 TargetScan、PITA、PACMIT 和 miRanda 的特征。我们的 miRmap 网络应用程序为浏览用于模式生物的预先计算的 miRNA 靶标预测提供了一个用户友好且功能丰富的资源，也为预测和对用户提交的序列进行排名提供了一个资源。MiRmap 网络集成了来自多个查询的排序、过滤和导出结果，并提供了编程访问，可在 http://mirmap.ezlab.org 上获得。
