Department of Plant and Soil Sciences , University of Delaware, Newark, Delaware 19711, USA.
Plant Physiol. 2013 Jul;162(3):1459-72. doi: 10.1104/pp.113.219162. Epub 2013 Jun 4.
Toll/interleukin receptor (TIR) domain-containing proteins encoded in the Arabidopsis (Arabidopsis thaliana) genome include the TIR-nucleotide binding site (TN) and TIR-unknown site/domain (TX) families. We investigated the function of these proteins. Transient overexpression of five TX and TN genes in tobacco (Nicotiana benthamiana) induced chlorosis. This induced chlorosis was dependent on ENHANCED DISEASE RESISTANCE1, a dependency conserved in both tobacco and Arabidopsis. Stable overexpression transgenic lines of TX and TN genes in Arabidopsis produced a variety of phenotypes associated with basal innate immune responses; these were correlated with elevated levels of salicylic acid. The TN protein AtTN10 interacted with the chloroplastic protein phosphoglycerate dehydrogenase in a yeast (Saccharomyces cerevisiae) two-hybrid screen; other TX and TN proteins interacted with nucleotide binding-leucine-rich repeat proteins and effector proteins, suggesting that TN proteins might act in guard complexes monitoring pathogen effectors.
拟南芥（Arabidopsis thaliana）基因组中编码的 toll/白细胞介素受体（TIR）域蛋白包括 TIR-核苷酸结合位点（TN）和 TIR-未知位点/结构域（TX）家族。我们研究了这些蛋白的功能。在烟草（Nicotiana benthamiana）中瞬时过表达五个 TX 和 TN 基因会诱导叶片黄化。这种黄化依赖于增强的疾病抗性 1（ENHANCED DISEASE RESISTANCE1），在烟草和拟南芥中都有这种依赖性。拟南芥中 TX 和 TN 基因的稳定过表达转基因株系产生了与基础先天免疫反应相关的多种表型；这些表型与水杨酸水平升高有关。TN 蛋白 AtTN10 在酵母（Saccharomyces cerevisiae）双杂交筛选中与质体蛋白磷酸甘油酸脱氢酶相互作用；其他 TX 和 TN 蛋白与核苷酸结合-富含亮氨酸重复蛋白和效应蛋白相互作用，表明 TN 蛋白可能在监视病原体效应子的防护复合物中发挥作用。
