School of Chemistry and Biochemistry and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA 30332-0400, USA.
Curr Opin Chem Biol. 2013 Aug;17(4):656-62. doi: 10.1016/j.cbpa.2013.05.019. Epub 2013 Jun 13.
Fluorescent probes are powerful and cost-effective tools for the detection of metal ions in biological systems. Compared to non-redox-active metal ions, the design of fluorescent probes for biological copper is challenging. Within the reducing cellular environment, copper is predominantly present in its monovalent oxidation state; therefore, the design of fluorescent probes for biological copper must take into account the rich redox and coordination chemistry of Cu(I). Recent progress in understanding the underlying solution chemistry and photophysical pathways led to the development of new probes that offer high fluorescence contrast and excellent selectivity towards monovalent copper.
荧光探针是用于检测生物体系中金属离子的强大且经济有效的工具。与非氧化还原活性金属离子相比，设计用于生物铜的荧光探针具有挑战性。在还原细胞环境中，铜主要以一价氧化态存在；因此，设计用于生物铜的荧光探针必须考虑到 Cu(I)丰富的氧化还原和配位化学。对基础溶液化学和光物理途径的理解的最新进展导致了新探针的发展，这些探针对一价铜具有高荧光对比度和优异的选择性。
