Magnarelli L A, Miller J N, Anderson J F, Riviere G R
Department of Entomology, Connecticut Agricultural Experiment Station, New Haven 06504.
J Clin Microbiol. 1990 Jun;28(6):1276-9. doi: 10.1128/jcm.28.6.1276-1279.1990.
Serum samples obtained from 59 persons who had acute necrotizing ulcerative gingivitis, periodontitis, syphilis, or Lyme disease were tested against Treponema phagedenis biotype Reiter, Treponema denticola, Treponema vincentii, and Treponema scoliodontum by indirect fluorescent-antibody staining methods. Although there were positive reactions for sera representing each of these study groups and for 20 (13%) of 156 samples collected from the general population (premarital screening for syphilis), titration endpoints were relatively low (less than or equal to 1:256). Serum samples from 18 persons who had gingivitis or periodontitis but no history of Lyme borreliosis were tested by enzyme-linked immunosorbent assay for antibodies to Borrelia burgdorferi. Of these, five (28%) had immunoglobulin M antibody and four (22%) contained immunoglobulin G antibodies to this spirochete. Adsorption with either sorbent commercially prepared from T. phagedenis biotype Reiter or with washed, whole cells of T. phagedenis biotype Reiter reduced cross-reactivity in the enzyme-linked immunosorbent assay for Lyme borreliosis.
采用间接荧光抗体染色法，对59例患有急性坏死性溃疡性龈炎、牙周炎、梅毒或莱姆病的患者的血清样本，检测其对具核梭杆菌生物变种赖特尔、齿垢密螺旋体、文森特密螺旋体和弯曲密螺旋体的反应。尽管这些研究组的血清样本以及从普通人群中采集的156份样本（梅毒婚前筛查）中的20份（13%）出现了阳性反应，但滴定终点相对较低（小于或等于1:256）。采用酶联免疫吸附测定法，对18例患有龈炎或牙周炎但无莱姆病螺旋体病病史的患者的血清样本检测抗伯氏疏螺旋体抗体。其中，5例（28%）含有抗该螺旋体的免疫球蛋白M抗体，4例（22%）含有免疫球蛋白G抗体。用从具核梭杆菌生物变种赖特尔商业化制备的吸附剂或具核梭杆菌生物变种赖特尔的洗涤全细胞进行吸附，可降低莱姆病螺旋体病酶联免疫吸附测定中的交叉反应。
