Centers for Disease Control and Prevention, Atlanta, GA, USA.
Instituto Nacional de Enfemedades Infecciosos C. Malbrán, Buenos Aires, Argentina.
J Gen Virol. 2013 Sep;94(Pt 9):2017-2028. doi: 10.1099/vir.0.053827-0. Epub 2013 Jun 26.
The family Picornaviridae is a large and diverse group of viruses that infect humans and animals. Picornaviruses are among the most common infections of humans and cause a wide spectrum of acute human disease. This study began as an investigation of acute flaccid paralysis (AFP) in a small area of eastern Bolivia, where surveillance had identified a persistently high AFP rate in children. Stools were collected and diagnostic studies ruled out poliovirus. We tested stool specimens from 51 AFP cases and 34 healthy household or community contacts collected during 2002-2003 using real-time and semi-nested reverse transcription polymerase chain reaction assays for enterovirus, parechovirus, cardiovirus, kobuvirus, salivirus and cosavirus. Anecdotal reports suggested a temporal association with neurological disease in domestic pigs, so six porcine stools were also collected and tested with the same set of assays, with the addition of an assay for porcine teschovirus. A total of 126 picornaviruses were detected in 73 of 85 human individuals, consisting of 53 different picornavirus types encompassing five genera (all except Kobuvirus). All six porcine stools contained porcine and/or human picornaviruses. No single virus, or combination of viruses, specifically correlated with AFP; however, the study revealed a surprising complexity of enteric picornaviruses in a single community.
家族小 RNA 病毒科是一组庞大且多样的病毒，感染人类和动物。小 RNA 病毒是人类最常见的感染之一，可引起广泛的急性人类疾病。本研究始于对玻利维亚东部一个小地区急性弛缓性麻痹（AFP）的调查，该地区的监测发现儿童 AFP 发病率持续居高不下。收集粪便，并通过诊断研究排除了脊髓灰质炎病毒。我们使用实时和半巢式逆转录聚合酶链反应检测法，对 2002 年至 2003 年间收集的 51 例 AFP 病例和 34 例健康家庭或社区接触者的粪便标本进行了检测，以检测肠道病毒、肠道病毒、心血管病毒、柯萨奇病毒、唾液病毒和冠状病毒。一些传闻报告表明，猪的神经系统疾病与 AFP 之间存在时间关联，因此还收集了六份猪粪便，并使用相同的检测方法进行了检测，同时增加了检测猪肠道病毒的检测方法。总共从 85 个人中的 73 个人中检测到 126 种小 RNA 病毒，包括五个属的 53 种不同小 RNA 病毒类型（除了柯萨奇病毒属）。所有六份猪粪便均含有猪和/或人类小 RNA 病毒。没有一种病毒或多种病毒与 AFP 有特定的相关性；然而，该研究揭示了单一社区中小肠 RNA 病毒的惊人复杂性。
