Virology and Experimental Therapy Laboratory, Aggeu Magalhães Research Center, Fiocruz, Avenida Moraes Rego s/n, Campus da UFPE, Cidade Universitária, 50670-420 Recife, PE, Brazil.
J Trop Med. 2013;2013:648475. doi: 10.1155/2013/648475. Epub 2013 Jun 2.
Human leukocyte antigen (HLA) alleles have been correlated with susceptibility or resistance to severe dengue; however, few immunogenetic studies have been performed in Latin American (LA) populations. We have conducted immunogenetic studies of HLA class I and II alleles in a cohort of 187 patients with DENV-3 infection and confirmed clinical diagnosis of either severe dengue, known as dengue hemorrhagic fever (DHF), or the less severe form, dengue fever (DF), in Recife, Pernambuco, Brazil. An association analysis was performed using Fisher's association test, with odds ratios (ORs) calculated using conditional maximum likelihood estimates. HLA-B∗44 (P = 0.047, OR = 2.025, 95% CI = 0.97-4.24) was found to be associated with increased susceptibility to DHF in response to DENV-3 infection. In addition, HLA-B∗07 (P = 0.048, OR = 0.501, one-sided 95% CI = 0-0.99) and HLA-DR∗13 (P = 0.028, OR = 0.511, one-sided 95% CI = 0-0.91) were found to be associated with resistance to secondary dengue infection by DENV-3. These results suggest that HLA-B∗44 supertype alleles and their respective T-cell responses might be involved in susceptibility to severe dengue infections, whereas the HLA-B∗07 supertype alleles and DR∗13 might be involved in cross-dengue serotype immunity.
人类白细胞抗原 (HLA) 等位基因与重症登革热的易感性或抗性相关；然而，在拉丁美洲 (LA) 人群中进行的免疫遗传研究较少。我们在巴西伯南布哥州累西腓的一个队列中进行了 HLA Ⅰ类和Ⅱ类等位基因的免疫遗传学研究，该队列由 187 例感染 DEN-3 的患者组成，其临床诊断均为重症登革热，即登革出血热 (DHF)，或较轻的形式，登革热 (DF)。使用 Fisher 关联检验进行关联分析，使用条件最大似然估计计算比值比 (OR)。发现 HLA-B∗44 (P = 0.047，OR = 2.025，95%CI = 0.97-4.24) 与 DEN-3 感染后 DHF 的易感性增加相关。此外，HLA-B∗07 (P = 0.048，OR = 0.501，单侧 95%CI = 0-0.99) 和 HLA-DR∗13 (P = 0.028，OR = 0.511，单侧 95%CI = 0-0.91) 与 DEN-3 引起的二次登革感染的抗性相关。这些结果表明，HLA-B∗44 超型等位基因及其各自的 T 细胞反应可能与重症登革热感染的易感性有关，而 HLA-B∗07 超型等位基因和 DR∗13 可能与交叉登革热血清型免疫有关。
