1Department of Nanobiomedical Science, WCU Research Center, Dankook University, South Korea.
J Biomater Appl. 2014 Mar;28(7):1079-84. doi: 10.1177/0885328213496486. Epub 2013 Jul 8.
Novel microcarriers consisting of calcium phosphate cement and alginate were prepared for use as three-dimensional scaffolds for the culture and expansion of cells that are effective for bone tissue engineering. The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization. Moreover, a porous structure could be easily created in the solid microcarriers by soaking the produced microcarriers in water and a subsequent freeze-drying process. Bone mineral-like apatite nanocrystallites were shown to rapidly develop on the calcium phosphate cement-alginate microcarriers under moist conditions due to the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite. Osteoblastic cells cultured on the microspherical scaffolds were proven to be viable, with an active proliferative potential during 14 days of culture, and their osteogenic differentiation was confirmed by the determination of alkaline phosphatase activity. The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
新型的磷酸钙水泥-海藻酸钠微载体被制备出来，用作细胞培养和扩增的三维支架，这对于骨组织工程来说是非常有效的。磷酸钙水泥-海藻酸钠复合微载体是通过将复合水溶液在油浴中按不同比例（磷酸钙水泥粉末/海藻酸钠溶液=0.8-1.2）乳化，然后在成球过程中使混合物原位硬化而制成的。此外，通过将制备的微载体浸泡在水中并随后进行冷冻干燥过程，可以很容易地在固体微载体中创建多孔结构。在潮湿条件下，磷酸钙水泥-海藻酸钠微载体上迅速形成类似于骨矿物质的磷灰石纳米晶，这是由于磷酸钙水泥中的α-磷酸三钙相转化为碳酸羟基磷灰石。在微球支架上培养的成骨细胞在培养的 14 天内具有活力和活跃的增殖潜力，其成骨分化通过碱性磷酸酶活性的测定得到证实。本文开发的原位硬化磷酸钙水泥-海藻酸钠微载体可用作细胞输送和骨组织工程的潜在三维支架。
