Department of Food Function and Labeling, National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, Japan ; Cooperative Major in Advanced Health Science, Tokyo University of Agriculture and Technology, 2-24-16 Nakamachi, Koganei, Tokyo 184-8588, Japan.
J Clin Biochem Nutr. 2013 Jul;53(1):41-8. doi: 10.3164/jcbn.12-123. Epub 2013 Jun 1.
S-equol is a natural metabolite of the soy isoflavone, daidzein, produced by intestinal bacteria. S-equol has been shown to have greater estrogenic activity than other soy isoflavones and prevent bone loss in post-menopausal women. Estrogen regulates both bone remodeling and hemopoiesis in the bone marrow, these processes that communicate closely with each other. In this study, we investigated the effect of S-equol on bone mass and gene expression of bone marrow cells in ovariectomized (OVX) mice. Female ddY strain mice, aged 12 weeks, were either sham operated or OVX. The OVX mice were randomly divided into two groups: (1) OVX control and (2) OVX fed a 0.06% (w/w) S-equol supplemented diet. After 2 weeks, the trabecular bone volume of the femoral distal metaphysis was markedly reduced in OVX mice. However, treatment with equol was observed to ameliorate this. Expression of inflammatory-, osteoclastogenesis- and adipogenesis-related genes was increased in OVX mice compared with sham mice, and equol was observed to suppress their expression. The present study demonstrates that equol might ameliorate bone loss caused by estrogen deficiency through regulating hemopoiesis and production of inflammatory cytokines in bone marrow cells.
S-雌马酚是大豆异黄酮，大豆甙元的一种天然代谢产物，由肠道细菌产生。S-雌马酚的雌激素活性大于其他大豆异黄酮，能预防绝经后妇女的骨质流失。雌激素调节骨髓中的骨重塑和造血过程，这些过程密切相关。在这项研究中，我们研究了 S-雌马酚对去卵巢（OVX）小鼠骨量和骨髓细胞基因表达的影响。12 周龄雌性 ddY 品系小鼠接受假手术或 OVX。OVX 小鼠随机分为两组：（1）OVX 对照组和（2）OVX 喂食 0.06%（w/w）S-雌马酚补充饮食。2 周后，OVX 小鼠的股骨远端干骺端小梁骨体积明显减少，但用雌马酚处理可改善这种情况。与假手术小鼠相比，OVX 小鼠的炎症、破骨细胞生成和脂肪生成相关基因表达增加，而雌马酚可抑制其表达。本研究表明，雌马酚可能通过调节骨髓细胞的造血和炎症细胞因子的产生来改善雌激素缺乏引起的骨质流失。
