Department of Food and Nutrition, Korea University, Seoul, 136-703, Republic of Korea.
J Sci Food Agric. 2014 Mar 30;94(5):850-6. doi: 10.1002/jsfa.6317. Epub 2013 Aug 19.
To improve the extraction and recovery of bioactive materials from cactus, the present study investigated the effect of polysaccharide-degrading enzymes [Rapidase-Viscozyme mixture, 1/3 (v/v)] treatment under high hydrostatic pressure (HHP).
The dry weight of the extract increased with the use of increasing pressure regardless of enzyme treatment. However, the polyphenol content showed a tendency to decrease with the increase in pressure in the cactus extract with or without enzyme treatment. The enzyme-assisted extraction resulted in an increase of dry weight and polyphenol content in the cactus extract. The total sugar and reducing sugar contents of the cactus extract increased with increasing pressure in enzyme-assisted extraction. The uronic acid content of the cactus extract showed a pattern similar to that of the reducing sugars. The enzyme-assisted extraction also increased the contents of taxifolin, quercetin and isorhametin. The cactus extract obtained through enzyme-assisted extraction showed intense scavenging activity of both DPPH and ABTS radicals. The crude polysaccharides isolated from the extract (51.2% at 1000 µg mL⁻¹ for HHP extraction at 300 MPa) had higher anti-complementary activity than the others except for lipopolysaccharide (60.00% at 1000 µg mL⁻¹). HHP extraction and enzyme-assisted extraction using HHP showed an increase of anti-complementary activity compared with the heat and enzyme controls, respectively.
Overall, the use of HHP in enzyme-assisted extraction resulted in more efficient extraction than the use of enzyme treatment alone.
为了提高仙人掌中生物活性物质的提取和回收效率，本研究探讨了多糖降解酶[Rapidase-Viscozyme 混合物，1/3（v/v）]在高静压（HHP）下处理的效果。
无论是否使用酶处理，随着压力的增加，提取物的干重均增加。然而，在有或没有酶处理的情况下，仙人掌提取物的多酚含量随着压力的增加呈现出下降的趋势。酶辅助提取导致仙人掌提取物的干重和多酚含量增加。在酶辅助提取中，随着压力的增加，仙人掌提取物中的总糖和还原糖含量增加。仙人掌提取物中的糖醛酸含量与还原糖的含量模式相似。酶辅助提取还增加了 taxifolin、槲皮素和异鼠李素的含量。通过酶辅助提取获得的仙人掌提取物对 DPPH 和 ABTS 自由基均具有强烈的清除活性。从提取物中分离得到的粗多糖（在 300 MPa 下，1000µg/mL 时的 HHP 提取为 51.2%）的抗补体活性高于其他物质，除脂多糖（在 1000µg/mL 时为 60.00%）外。与热和酶对照相比，HHP 提取和使用 HHP 的酶辅助提取均显示出抗补体活性的增加。
总的来说，与单独使用酶处理相比，HHP 辅助酶提取的使用导致更有效的提取。
