Meyerhoff D J, Karczmar G S, Matson G B, Boska M D, Weiner M W
Magnetic Resonance Unit, Veterans Administration Medical Center, San Francisco, CA.
NMR Biomed. 1990 Feb;3(1):17-22. doi: 10.1002/nbm.1940030103.
Phosphorus-containing metabolites in normal human liver have been quantitated non-invasively with 31P magnetic resonance spectroscopy using surface coils. The location of the volume of interest (VOI) was defined by 1H magnetic resonance imaging. Subsequently, a modified three-dimensional localization technique (ISIS) was used to acquire 31P magnetic resonance spectra from the VOI. To account for partial saturation produced by rapid signal averaging, the spin/lattice relaxation times (T1) of all hepatic phosphorus resonances were measured. The corrected resonance integrals were used to derive absolute molar concentrations for the following hepatic metabolites (mmol/kg wet weight): ATP, 2.0; inorganic phosphate, 2.1; phosphodiesters, 5.4; and phosphomonoesters, 0.9. These values are compared with previously reported values for humans using freeze-clamping techniques, and provide a basis for comparison with studies of hepatic disease in this laboratory.
使用表面线圈，通过31P磁共振波谱法对正常人体肝脏中含磷代谢物进行了无创定量分析。感兴趣体积（VOI）的位置通过1H磁共振成像确定。随后，采用改进的三维定位技术（ISIS）从VOI获取31P磁共振波谱。为了考虑快速信号平均产生的部分饱和，测量了所有肝脏磷共振的自旋/晶格弛豫时间（T1）。校正后的共振积分用于推导以下肝脏代谢物的绝对摩尔浓度（mmol/kg湿重）：ATP，2.0；无机磷酸盐，2.1；磷酸二酯，5.4；磷酸单酯，0.9。将这些值与先前使用冷冻钳夹技术报告的人类值进行比较，并为本实验室肝病研究的比较提供了基础。
