使用靶向 αvβ3 整合素的微球体的磁动力光学相干断层扫描评估动脉粥样硬化病变。
Magnetomotive optical coherence tomography for the assessment of atherosclerotic lesions using αvβ3 integrin-targeted microspheres.
机构信息
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 North Mathews Avenue, Urbana, IL, 61801, USA.
出版信息
Mol Imaging Biol. 2014 Feb;16(1):36-43. doi: 10.1007/s11307-013-0671-6.
PURPOSE
We investigated the early-stage fatty streaks/plaques detection using magnetomotive optical coherence tomography (MM-OCT) in conjunction with αvβ3 integrin-targeted magnetic microspheres (MSs). The targeting of functionalized MSs was investigated by perfusing ex vivo aortas from an atherosclerotic rabbit model in a custom-designed flow chamber at physiologically relevant pulsatile flow rates and pressures.
PROCEDURES
Aortas were extracted and placed in a flow chamber. Magnetic MS contrast agents were perfused through the aortas and MM-OCT, fluorescence confocal, and bright field microscopy were performed on the ex vivo aorta specimens for localizing the MSs.
RESULTS
The results showed a statistically significant and stronger MM-OCT signal (3.30 ± 1.73 dB) from the aorta segment perfused with targeted MSs, compared with the nontargeted MSs (1.18 ± 0.94 dB) and control (0.78 ± 0.41 dB) aortas. In addition, there was a good co-registration of MM-OCT signals with confocal microscopy.
CONCLUSIONS
Early-stage fatty streaks/plaques have been successfully detected using MM-OCT in conjunction with αvβ3 integrin-targeted magnetic MSs.
目的
我们使用磁动力光相干断层扫描（MM-OCT）结合 αvβ3 整合素靶向磁性微球（MS）来研究早期脂肪条纹/斑块的检测。通过在定制的流动室中以生理相关的脉动流速和压力从动脉粥样硬化兔模型中灌注离体主动脉，研究了功能化 MS 的靶向作用。
程序
提取主动脉并放置在流动室内。将磁性 MS 造影剂灌注通过主动脉，对离体主动脉标本进行 MM-OCT、荧光共聚焦和明场显微镜检查，以定位 MS。
结果
结果显示，与非靶向 MS（1.18 ± 0.94 dB）和对照（0.78 ± 0.41 dB）主动脉相比，用靶向 MS 灌注的主动脉段的 MM-OCT 信号要强得多（3.30 ± 1.73 dB）。此外，MM-OCT 信号与共聚焦显微镜有很好的配准。
结论
使用 MM-OCT 结合 αvβ3 整合素靶向磁性 MS 成功检测到早期的脂肪条纹/斑块。
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