AIT Austrian Institute of Technology GmbH, Bioresources Unit, Tulln, Austria.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4586-4590. doi: 10.1099/ijs.0.052654-0. Epub 2013 Aug 1.
A Gram-reaction-negative, yellow-pigmented strain, designated EX36(T), was characterized using a polyphasic approach comprising phylogenetic, morphological and genotypic analyses. The endophytic strain was isolated from Zn/Cd-accumulating Salix caprea in Arnoldstein, Austria. Analysis of the 16S rRNA gene demonstrated that the novel strain is most closely related to members of the genus Spirosoma (95 % sequence similarity with Spirosoma linguale). The genomic DNA G+C content was 47.2 mol%. The predominant quinone was and the major cellular fatty acids were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), C16 : 1ω5c, iso-C17 : 0 3-OH and iso-C15 : 0. On the basis of its phenotypic and genotypic properties, strain EX36(T) should be classified as a novel species of the genus Spirosoma, for which the name Spirosoma endophyticum sp. nov. is proposed. The type strain is EX36(T) ( = DSM 26130(T) = LMG 27272(T)).
一株革兰氏反应阴性、呈黄色的菌株，被命名为 EX36(T)，通过包括系统发育、形态和基因型分析的多相方法进行了特征描述。该内生菌株是从奥地利阿诺德斯泰因的 Zn/Cd 积累柳属植物中分离出来的。16S rRNA 基因分析表明，该新菌株与 Spirosoma 属的成员最为密切相关（与 Spirosoma linguale 的序列相似性为 95%）。基因组 DNA G+C 含量为 47.2 mol%。主要醌为 Q-10，主要细胞脂肪酸为 sumed feature 3（iso-C15:0 2-OH 和/或 C16:1ω7c）、C16:1ω5c、iso-C17:0 3-OH 和 iso-C15:0。基于其表型和基因型特性，菌株 EX36(T) 应被归类为 Spirosoma 属的一个新种，建议将其命名为 Spirosoma endophyticum sp. nov.。模式菌株为 EX36(T)（=DSM 26130(T)=LMG 27272(T)）。
