Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany.
Mater Sci Eng C Mater Biol Appl. 2013 Oct;33(7):3677-87. doi: 10.1016/j.msec.2013.04.058. Epub 2013 May 4.
In this study biomimetic poly(glycerol sebacate) PGS matrix was developed for cardiac patch application. The rationale was that such matrices would provide conducive environment for the seeded cells at the interphase with PGS. From the microstructural standpoint, PGS was fabricated into dense films and porous PGS scaffolds. From the biological aspect, biomimetic PGS membranes were developed via covalently binding peptides Tyr-Ile-Gly-Ser-Arg (YIGSR) and Gly-Arg-Gly-Asp-Ser-Pro (GRGDSP), corresponding to the epitope sequences of laminin and fibronectin, respectively onto the surface. To improve and enhance homogenous binding of peptides onto the PGS surface, chemical modification of its surface was carried out. A sequential regime of alkaline hydrolysis with 0.01 M NaOH for 5 min and acidification with 0.01 M HCl for 25s was optimal. More COOH chemical group was exposed without causing deleterious effect on the bulk properties of the polymer as revealed by the physicochemical analysis carried out. HPLC analysis, chemical imaging and ToF-SIMS were able to establish the successful homogenous functionalization of PGS membranes with the peptides. Finally, the developed biomimetic membranes supported the adhesion and growth of rat and human cardiac progenitor cells.
本研究开发了仿生聚（甘油癸二酸酯）（PGS）基质，用于心脏贴片应用。其原理是，此类基质将在与 PGS 的相间为接种细胞提供有利环境。从微观结构的角度来看，将 PGS 制成致密膜和多孔 PGS 支架。从生物学角度来看，通过将对应于层粘连蛋白和纤连蛋白的表位序列的 Tyr-Ile-Gly-Ser-Arg（YIGSR）和 Gly-Arg-Gly-Asp-Ser-Pro（GRGDSP）肽共价结合到表面上来开发仿生 PGS 膜。为了提高和增强肽在 PGS 表面上的均匀结合，对其表面进行了化学修饰。用 0.01 M NaOH 碱水解 5 分钟，然后用 0.01 M HCl 酸化 25 秒是最佳方案。如通过所进行的物理化学分析所揭示的，更多的 COOH 化学基团暴露出来，而不会对聚合物的本体性质造成有害影响。HPLC 分析、化学成像和 ToF-SIMS 能够证明肽在 PGS 膜上的成功均匀功能化。最后，开发的仿生膜支持大鼠和人心肌祖细胞的黏附和生长。
