Department of Gastrointestinal Microbiology, German Institute of Human Nutrition Potsdam-Rehbrücke (DIfE), Nuthetal, Germany.
ZIEL-Research Center for Nutrition and Food Sciences, Junior Research Group Intestinal Microbiome, Biofunctionality Unit, Technische Universität München (TUM), Freising, Germany.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4606-4612. doi: 10.1099/ijs.0.051441-0. Epub 2013 Aug 5.
A Gram-positive, spore-forming, non-motile, strictly anaerobic rod-shaped bacterium was isolated from the caecal content of a TNF(deltaARE) mouse. The isolate, referred to as strain SRB-521-5-I(T), was originally cultured on a reduced agar medium containing yeast extract, rumen fluid and lactic acid as main energy and carbon sources. Phylogenetic analysis of partial 16S rRNA genes revealed that the species most closely related to strain SRB-521-5-I(T) were Flavonifractor plautii and Pseudoflavonifractor capillosus (<95 % sequence similarity; 1436 bp). In contrast to F. plautii and P. capillosus, strain SRB-521-5-I(T) contained a substantial amount of C18 : 0 dimethylacetal. Additional major fatty acids were C14 : 0 methyl ester, C16 : 0 dimethylacetal and C18 : 0 aldehyde. Strain SRB-521-5-I(T) differed in its enzyme profile from F. plautii and P. capillosus by being positive for dextrin, maltotriose, turanose, dl-lactic acid and d-lactic acid methyl ester but negative for d-fructose. In reduced Wilkins-Chalgren-Anaerobe broth, strain SRB-521-5-I(T) produced approximately 8 mM butyrate and 4 mM acetate. In contrast to F. plautii, the strain did not metabolize flavonoids. It showed intermediate resistance towards the antibiotics ciprofloxacin, colistin and tetracycline. Based on genotypic and phenotypic characteristics, we propose the name Intestinimonas butyriciproducens gen. nov., sp. nov. to accommodate strain SRB-521-5-I(T) ( = DSM 26588(T) = CCUG 63529(T)) as the type strain.
从 TNF(deltaARE) 小鼠的盲肠内容物中分离到一株革兰氏阳性、产孢子、非运动、严格厌氧的杆状细菌。该分离株被称为 SRB-521-5-I(T) 株，最初是在含有酵母提取物、瘤胃液和乳酸作为主要能源和碳源的还原琼脂培养基上培养的。部分 16S rRNA 基因的系统发育分析表明，与 SRB-521-5-I(T) 株最密切相关的物种是 Flavonifractor plautii 和 Pseudoflavonifractor capillosus(<95%序列相似性；1436 bp)。与 F. plautii 和 P. capillosus 不同，SRB-521-5-I(T)株含有大量的 C18:0 二甲缩醛。其他主要脂肪酸是 C14:0 甲酯、C16:0 二甲缩醛和 C18:0 醛。与 F. plautii 和 P. capillosus 相比，SRB-521-5-I(T)株在酶谱上的不同之处在于它对糊精、麦芽三糖、棉子糖、dl-乳酸和 d-乳酸甲酯呈阳性，但对 d-果糖呈阴性。在还原 Wilkins-Chalgren-Anaerobe 肉汤中，SRB-521-5-I(T)株产生约 8 mM 丁酸和 4 mM 乙酸。与 F. plautii 不同的是，该菌株不能代谢类黄酮。它对环丙沙星、粘菌素和四环素等抗生素表现出中等抗性。基于基因型和表型特征，我们建议将 Intestinimonas butyriciproducens gen. nov., sp. nov. 命名为新属新种，以容纳 SRB-521-5-I(T) 株( = DSM 26588(T) = CCUG 63529(T))作为模式株。
