MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, Scotland, United Kingdom.
Curr Opin Genet Dev. 2013 Oct;23(5):504-11. doi: 10.1016/j.gde.2013.06.003. Epub 2013 Aug 7.
Pluripotency is a property that early embryonic cells possess over a considerable developmental time span. Accordingly, pluripotent cell lines can be established from the pre-implantation or post-implantation mouse embryo as embryonic stem (ES) or epiblast stem (EpiSC) cell lines, respectively. Maintenance of the pluripotent phenotype depends on the function of specific transcription factors (TFs) operating within a pluripotency gene regulatory network (PGRN). As cells move from an ES cell to an EpiSC state, the PGRN changes with expression of some TFs reduced (e.g. Nanog) or eliminated (e.g. Esrrb). Re-expressing such TFs can move cells back to an earlier developmental identity and is being applied to attempt establishment of human cell lines with the properties of mouse ES cells.
多能性是早期胚胎细胞在相当长的发育时间跨度内所具有的一种特性。因此，可以分别从着床前或着床后小鼠胚胎中建立多能细胞系，作为胚胎干细胞 (ES) 或上胚层干细胞 (EpiSC) 细胞系。多能表型的维持依赖于特定转录因子 (TFs) 在多能基因调控网络 (PGRN) 中的功能。当细胞从 ES 细胞向 EpiSC 状态移动时，PGRN 会发生变化，一些 TFs 的表达减少（例如 Nanog）或消除（例如 Esrrb）。重新表达这些 TF 可以使细胞回到更早的发育状态，并且正在被应用于尝试建立具有小鼠 ES 细胞特性的人类细胞系。
