The Second Clinical Medical College, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China ; Department of Acupuncture and Moxibustion, Zhongda Hospital Affiliated to Southeast University, Nanjing 210029, China.
Evid Based Complement Alternat Med. 2013;2013:871704. doi: 10.1155/2013/871704. Epub 2013 Jul 1.
Objectives. To compare the effects of moxibustion at two different temperatures (38°C and 46°C) on the blood cholesterol level in a mice model of acute hyperlipidemia, to detect the different expression levels of transient receptor potential vanilloid subfamily 1 (TRPV1) in the dorsal root ganglions of the wild mice, and to explore the correlation between TRPV1 and moxibustion's cholesterol-lowering effects. Method. Two different mice models were used: C57BL/6J wild type (WT) and TRPV1 gene knockout (TRPV1-/-). Each model was randomly divided into control group and model group with three subgroups after acute hyperlipidemia was established: model control group, 38°C moxibustion group, and 46°C moxibustion group. The mice in 38°C group and 46°C group were subject to moxibustion. After the therapy, the cholesterol concentration in serum was measured, and the expression of TRPV1 was quantified. Results. In WT mice, moxibustion caused a decrease in blood cholesterol level and upregulation of TRPV1 at the mRNA level, which was significantly greater in the 46°C group. In contrast, in TRPV1-/- mice, the differences of cholesterol-lowering effects of moxibustion were lost. Conclusions. Temperature is one of the important factors affecting the effects of moxibustion, and the cholesterol -lowering effect of moxibustion is related to the activation of TRPV1.
目的。比较两种不同温度（38°C 和 46°C）艾灸对急性高脂血症模型小鼠血胆固醇水平的影响，检测野生型小鼠背根神经节中瞬时受体电位香草酸亚型 1（TRPV1）的不同表达水平，并探讨 TRPV1 与艾灸降胆固醇作用的相关性。方法。使用两种不同的小鼠模型：C57BL/6J 野生型（WT）和 TRPV1 基因敲除（TRPV1-/-）。急性高脂血症建立后，每个模型随机分为对照组和模型组，每组又分为三个亚组：模型对照组、38°C 艾灸组和 46°C 艾灸组。38°C 组和 46°C 组进行艾灸。治疗后，检测血清胆固醇浓度，定量分析 TRPV1 的表达。结果。在 WT 小鼠中，艾灸可降低血胆固醇水平，并上调 TRPV1 的 mRNA 水平，在 46°C 组更为显著。相比之下，在 TRPV1-/-小鼠中，艾灸的降胆固醇作用差异消失。结论。温度是影响艾灸效果的重要因素之一，艾灸的降胆固醇作用与 TRPV1 的激活有关。
