Al-Farabi Kazakh National University, Almaty 050038, Kazakhstan.
Biomed Res Int. 2013;2013:307145. doi: 10.1155/2013/307145. Epub 2013 Jul 11.
We identified the interaction sites of several miRNAs with the mRNAs from paralogs and orthologs of the SPL and HAM genes in A. thaliana. miRNAs from the miR156 and miR157 families in A. thaliana are shown to have binding sites within the mRNAs of SPL genes. The ath-miR156a-j binding sites located in the mRNAs of the SPL paralogs contain the sequence GUGCUCUCUCUCUUCUGUCA. This sequence encodes the ALSLLS motif. miR157a-d bind to mRNAs of the SPL family at the same site. We suggest merging the miR156 and miR157 families into one family. Several SPL genes in eight plants contain conserved miR156 binding sites. GUGCUCUCUCUCUUCUGUCA polynucleotide is homologous in its binding sites. The ALSLLS hexapeptide is also conserved in the SPL proteins from these plants. Binding sites for ath-miR171a-c and ath-miR170 in HAM1, HAM2, and HAM3 paralog mRNAs are located in the CDSs. The conserved miRNA binding sequence GAUAUUGGCGCGGCUCAAUCA encodes the ILARLN hexapeptide. Nucleotides within the HAM1, HAM2, and HAM3 miRNA binding sites are conserved in the mRNAs of 37 orthologs from 13 plants. The miR171- and miR170-binding sites within the ortholog mRNAs were conserved and encode the ILARLN motif. We suggest that the ath-miR170 and ath-miR171a-c families should be in one family.
我们鉴定了几个 miRNA 与拟南芥 SPL 和 HAM 基因的同源物和同系物 mRNA 的相互作用位点。在拟南芥中，miR156 和 miR157 家族的 miRNA 被发现有结合位点位于 SPL 基因的 mRNA 内。位于 SPL 同源物 mRNA 中的 ath-miR156a-j 结合位点包含 GUGCUCUCUCUCUUCUGUCA 序列。该序列编码 ALSLLS 基序。miR157a-d 在相同的位点与 SPL 家族的 mRNA 结合。我们建议将 miR156 和 miR157 家族合并为一个家族。八个植物中的几个 SPL 基因含有保守的 miR156 结合位点。在其结合位点中，GUGCUCUCUCUCUUCUGUCA 多核苷酸是同源的。这些植物中的 SPL 蛋白中的 ALSLLS 六肽也是保守的。ath-miR171a-c 和 ath-miR170 在 HAM1、HAM2 和 HAM3 同源物 mRNA 中的结合位点位于 CDS 内。保守的 miRNA 结合序列 GAUAUUGGCGCGGCUCAAUCA 编码 ILARLN 六肽。HAM1、HAM2 和 HAM3 miRNA 结合位点内的核苷酸在来自 13 种植物的 37 个同源物的 mRNA 中是保守的。在同源物 mRNA 中的 miR171 和 miR170 结合位点是保守的，并编码 ILARLN 基序。我们建议 ath-miR170 和 ath-miR171a-c 家族应该在一个家族中。
