Department of Dermatology and Venereology, Medical University of Lodz, 5 Krzemieniecka Street, 94-017 Lodz, Poland.
Mediators Inflamm. 2013;2013:967987. doi: 10.1155/2013/967987. Epub 2013 Jul 21.
Dermatitis herpetiformis (DH) and bullous pemphigoid (BP) are skin diseases associated with eosinophilic and neutrophilic infiltrations. Although cytokines are critical for the inflammatory process, there are single findings concerning concentration of IL-17 in bullous diseases. The goal of this study was to assess IL-17 expression in DH and BP patients. Skin biopsies were taken from 10 DH, 14 BP patients and from 10 healthy subjects. The localization and expression of IL-17 was studied by immunohistochemistry and the serum concentration was measured by immunoassays. Expression of IL-17 in the epidermis and in influxed cells in dermis was detected in skin biopsies. Expression of IL-17 was statistically higher in epidermis and infiltration cells in specimens from BP than from DH patients. Examined interleukin expression was detected in perilesional skin of all patients but it was much lower than in lesional skin. The expression of IL-17 was not observed in biopsies from healthy people. Serum level of IL-17 was statistically higher in BP and DH groups as compared to control group. Our results provide the evidence that IL-17 may play an essential role in activating and recruiting eosinophils and neutrophils, which ultimately contribute to the tissue damage in DH and BP.
疱疹样皮炎（DH）和大疱性类天疱疮（BP）是与嗜酸性粒细胞和中性粒细胞浸润相关的皮肤疾病。尽管细胞因子对于炎症过程至关重要，但在大疱性疾病中，关于 IL-17 浓度的单一发现仍然很少。本研究的目的是评估 DH 和 BP 患者中 IL-17 的表达。从 10 名 DH 患者、14 名 BP 患者和 10 名健康受试者中采集皮肤活检。通过免疫组织化学研究 IL-17 的定位和表达，并通过免疫测定法测量血清浓度。在皮肤活检中检测到表皮和真皮中浸润细胞的 IL-17 表达。与 DH 患者相比，BP 患者的表皮和浸润细胞中 IL-17 的表达在统计学上更高。在所有患者的皮损周围皮肤中均检测到检查的白细胞介素表达，但低于皮损皮肤中的表达。在来自健康人的活检中未观察到 IL-17 的表达。与对照组相比，BP 和 DH 组的血清 IL-17 水平在统计学上更高。我们的研究结果提供了证据，表明 IL-17 可能在激活和招募嗜酸性粒细胞和中性粒细胞方面发挥重要作用，而这些细胞最终导致 DH 和 BP 中的组织损伤。
