Thent Zar Chi, Lin Teoh Seong, Das Srijit, Zakaria Zaiton
Department of Anatomy, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abd Aziz, 50300, Kuala Lumpur, Malaysia.
Afr J Tradit Complement Altern Med. 2012 Apr 2;9(3):396-404. eCollection 2012.
Cardiovascular complications are one of the major causes of death in diabetes mellitus. Piper sarmentosum (P.s) is an herb that possesses antihyperglycaemic effects. The main aim of the study was to observe the histological changes in the heart and the proximal aorta of streptozotocin-induced diabetic rats following P.s administration. Twenty-four male Sprague-Dawley rats (n=24) were equally randomized into four groups: control group supplemented with normal saline (C); control group supplemented with P.s (CTx) ; diabetic group supplemented with normal saline (D) and, diabetic group supplemented with P.s (DTx). Diabetes was induced by STZ (50mg/kg body weight) intramuscularly. P.s extract (0.125g/kg) was administered orally for 28 days, following four weeks of STZ induction. The cardiac and aortic tissues were collected and processed under different stains: Haematoxylin and Eosin (H & E), Verhoeff-Van Gieson (VVG), Masson's Trichome (MT) and Periodic Acid- Schiff (PAS). There were abnormal cardiomyocytes nuclei, disarray of myofibres and increase in connective tissue deposits in cardiac tissues of the diabetic untreated group. The thickness of tunica media and ratio of tunica intima to media were found to be significantly increased in the aorta of diabetic untreated group (P < 0.05) compared to the control group. There were degenerative changes in the proximal aorta in diabetic untreated groups. All the histological damages of cardiac and aortic tissues were found to be lesser in the diabetic treated groups. Supplementation with P.s extract prevented the oxidative damage arising from diabetes mellitus, and reduced its complications.
心血管并发症是糖尿病患者主要的死亡原因之一。蒌叶是一种具有降血糖作用的草药。本研究的主要目的是观察给予蒌叶后链脲佐菌素诱导的糖尿病大鼠心脏和主动脉近端的组织学变化。将24只雄性Sprague-Dawley大鼠（n = 24）平均随机分为四组：补充生理盐水的对照组（C）；补充蒌叶的对照组（CTx）；补充生理盐水的糖尿病组（D）；补充蒌叶的糖尿病组（DTx）。通过肌肉注射链脲佐菌素（50mg/kg体重）诱导糖尿病。在链脲佐菌素诱导四周后，口服给予蒌叶提取物（0.125g/kg），持续28天。收集心脏和主动脉组织，并进行不同染色处理：苏木精和伊红染色（H&E）、Verhoeff-Van Gieson染色（VVG）、Masson三色染色（MT）和过碘酸-希夫染色（PAS）。未治疗的糖尿病组心脏组织中存在心肌细胞核异常、肌纤维排列紊乱以及结缔组织沉积增加的情况。与对照组相比，未治疗的糖尿病组主动脉中膜厚度和内膜与中膜比值显著增加（P < 0.05）。未治疗的糖尿病组主动脉近端存在退行性改变。在接受治疗的糖尿病组中，心脏和主动脉组织的所有组织学损伤均较轻。补充蒌叶提取物可预防糖尿病引起的氧化损伤，并减少其并发症。
