Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia.
Lipids Health Dis. 2010 Apr 30;9:44. doi: 10.1186/1476-511X-9-44.
Piper sarmentosum (P.s) has flavonoid component in its leaves which has antioxidative effect. To date, its effect on atherosclerosis has not been studied histologically.
The study aimed to investigate the effect of P.s on atherosclerotic changes in hypercholesterolemic rabbits.
Forty two male New Zealand white rabbits were divided into seven groups. C - control group fed normal rabbit chow, CH - cholesterol diet (1% cholesterol), W1 - 1% cholesterol with water extract of P.s (62.5 mg/kg), W2 - 1% cholesterol with water extract of P.s (125 mg/kg), W3 - 1% cholesterol with water extract of P.s (250 mg/kg), W4 - 1% cholesterol with water extract of P.s (500 mg/kg) and Smv - 1% cholesterol supplemented with simvistatin drug (1.2 mg/kg). All rabbits were treated for 10 weeks. Following 10 weeks of supplementation, the animals were sacrificed and the aortic tissue was taken for histological study.
Rabbits fed only with high cholesterol diet 1% cholesterol (CH) showed focal fatty streak lesions compared to the C group and 1% cholesterol supplemented with simvistatin drug (Smv) group. Atherosclerotic lesions in the 1% cholesterol group supplemented with P.s (500 mg/kg) i.e. W4 group showed significant reduction (30 + or - 6.0%, p < 0.05) in fatty streak compared to the high cholesterol group (85.6 + or - 4.1%) under Sudan IV stain. The atherosclerotic lesions under transmission electron microscope showed reduction in foam cells in the treatment groups compared to the CH groups.
Administration of P.s extract has protective effect against atheroscleros.
佩兰（P.s）的叶子含有类黄酮成分，具有抗氧化作用。迄今为止，尚未对其在动脉粥样硬化中的作用进行组织学研究。
本研究旨在探讨佩兰水提物对高胆固醇血症兔动脉粥样硬化病变的影响。
将 42 只雄性新西兰白兔分为 7 组。C-对照组给予正常兔饲料，CH-给予胆固醇饮食（1%胆固醇），W1-给予 1%胆固醇加佩兰水提取物（62.5mg/kg），W2-给予 1%胆固醇加佩兰水提取物（125mg/kg），W3-给予 1%胆固醇加佩兰水提取物（250mg/kg），W4-给予 1%胆固醇加佩兰水提取物（500mg/kg），Smv-给予 1%胆固醇加辛伐他汀药物（1.2mg/kg）。所有兔子均治疗 10 周。补充 10 周后，处死动物并取主动脉组织进行组织学研究。
仅给予 1%胆固醇高胆固醇饮食的兔子（CH）与 C 组和 1%胆固醇加辛伐他汀药物（Smv）组相比，出现局灶性脂肪条纹病变。与高胆固醇组（85.6+/-4.1%）相比，1%胆固醇加 500mg/kg 佩兰水提取物（W4 组）的动脉粥样硬化病变苏丹 IV 染色显示脂肪条纹明显减少（30+/-6.0%，p<0.05）。透射电镜下的动脉粥样硬化病变显示，与 CH 组相比，治疗组泡沫细胞减少。
佩兰提取物的给药具有抗动脉粥样硬化作用。
