Rahman M A, Begum M F, Alam M F
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.
Mycobiology. 2009 Dec;37(4):277-85. doi: 10.4489/MYCO.2009.37.4.277. Epub 2009 Dec 31.
In this study, dual culture, poison agar, and direct methods were used to assess the ability of Trichoderma virens IMI-392430, T. pseudokoningii IMI-392431, T. harzianum IMI-392432, T. harzianum IMI-392433, and T. harzianum IMI-392434 to control Ceratocystis paradoxa, which causes the pineapple disease of sugarcane. The highest percentage inhibition of radial growth (PIRG) values were observed with T. harzianum IMI-392432 using two dual culture methods, 63.80% in Method I and 80.82% in Method II. The minimum colony overgrowth time was observed with T. harzianum IMI-392432 and the maximum was observed with T. pseudokoningii IMI-392431. Different concentrations of different day-old metabolites of Trichoderma isolates were tested against mycelial growth of C. paradoxa. The highest PIRG (84.685%) exhibited at 80% concentration of 30-day-old metabolites of T. harzianum IMI-392432 using the modified bilayer poison agar method. In the direct assay method the maximum mycelial growth weight (PIGW) was observed at the same concentration and the same day-old metabolites of T. harzianum IMI-392432. This study showed that Trichoderma isolates have a good antagonistic effect on C. paradoxa mycelial growth and T. harzianum IMI-392432 has the most potential to control the pineapple disease pathogen.
在本研究中，采用双培养法、毒饵法和直接法评估了哈茨木霉IMI-392430、拟康氏木霉IMI-392431、哈茨木霉IMI-392432、哈茨木霉IMI-392433和哈茨木霉IMI-392434对引起甘蔗菠萝病的奇异长喙壳菌的防治能力。使用两种双培养法时，哈茨木霉IMI-392432的径向生长抑制率（PIRG）最高，方法I为63.80%，方法II为80.82%。哈茨木霉IMI-392432的菌落过度生长时间最短，拟康氏木霉IMI-392431的最长。测试了木霉菌株不同日龄代谢产物的不同浓度对奇异长喙壳菌菌丝生长的影响。使用改良双层毒饵法时，哈茨木霉IMI-392432 30日龄代谢产物浓度为80%时，径向生长抑制率最高（84.685%）。在直接测定法中，哈茨木霉IMI-392432相同浓度和相同日龄的代谢产物下，菌丝生长重量（PIGW）最大。本研究表明，木霉菌株对奇异长喙壳菌菌丝生长具有良好的拮抗作用，哈茨木霉IMI-392432在防治菠萝病病原菌方面最具潜力。
