Robinson H L, Zinkus D M
Department of Pathology, University of Massachusetts Medical Center, Worcester 01655.
J Virol. 1990 Oct;64(10):4836-41. doi: 10.1128/JVI.64.10.4836-4841.1990.
Human immunodeficiency virus type 1 DNA synthesis was followed in a CD4+ line of T cells (C8166) grown in the presence or absence of a monoclonal antibody to CD4 that blocks infection By 48 h after infection, cultures grown in the presence of the antibody contained approximately 4 copies of human immunodeficiency virus type 1 DNA per cell, whereas those grown in the absence of the antibody contained approximately 80 copies of viral DNA per cell. Most of the viral DNA in cultures grown in the absence of the antibody was present in a broad smear of apparently incomplete viral sequences. In cultures grown in the presence or absence of the antibody, the 9.6-kilobase linear duplex of viral DNA appeared to undergo integration within 24 h of its appearance. These results demonstrate that T cells accumulate unintegrated human immunodeficiency virus type 1 DNA as a result of multiple virions entering cells.
在存在或不存在阻断感染的抗CD4单克隆抗体的情况下，对在CD4 + T细胞系（C8166）中生长的1型人类免疫缺陷病毒DNA合成进行了跟踪。感染后48小时，在抗体存在下培养的细胞中，每个细胞含有约4份1型人类免疫缺陷病毒DNA，而在无抗体情况下培养的细胞中，每个细胞含有约80份病毒DNA。在无抗体培养的细胞中，大部分病毒DNA以明显不完整病毒序列的宽条带形式存在。在有或无抗体培养的细胞中，病毒DNA的9.6千碱基线性双链体在出现后24小时内似乎发生了整合。这些结果表明，由于多个病毒粒子进入细胞，T细胞积累了未整合的1型人类免疫缺陷病毒DNA。
