Department of Food Science, Cornell University, Ithaca, NY 14853, USA.
Food Microbiol. 2013 Dec;36(2):275-85. doi: 10.1016/j.fm.2013.06.014. Epub 2013 Jul 4.
Salmonella is an animal and human pathogen of worldwide concern. Surveillance programs indicate that the incidence of Salmonella serovars fluctuates over time. While bacteriophages are likely to play a role in driving microbial diversity, our understanding of the ecology and diversity of Salmonella phages is limited. Here we report the isolation of Salmonella phages from manure samples from 13 dairy farms with a history of Salmonella presence. Salmonella phages were isolated from 10 of the 13 farms; overall 108 phage isolates were obtained on serovar Newport, Typhimurium, Dublin, Kentucky, Anatum, Mbandaka, and Cerro hosts. Host range characterization found that 51% of phage isolates had a narrow host range, while 49% showed a broad host range. The phage isolates represented 65 lysis profiles; genome size profiling of 94 phage isolates allowed for classification of phage isolates into 11 groups with subsequent restriction fragment length polymorphism analysis showing considerable variation within a given group. Our data not only show an abundance of diverse Salmonella phage isolates in dairy farms, but also show that phage isolates that lyse the most common serovars causing salmonellosis in cattle are frequently obtained, suggesting that phages may play an important role in the ecology of Salmonella on dairy farms.
沙门氏菌是一种对动物和人类具有全球影响的病原体。监测计划表明，沙门氏菌血清型的发病率随时间波动。虽然噬菌体可能在驱动微生物多样性方面发挥作用，但我们对沙门氏菌噬菌体的生态学和多样性的了解有限。在这里，我们报告了从 13 个有沙门氏菌存在史的奶牛场的粪便样本中分离出沙门氏菌噬菌体。从 13 个农场中的 10 个农场中分离出了沙门氏菌噬菌体；总共从纽波特、肠炎、都柏林、肯塔基、阿纳图姆、姆班达卡和塞罗宿主中获得了 108 个噬菌体分离物。宿主范围特征表明，51%的噬菌体分离物具有窄宿主范围，而 49%的噬菌体分离物具有宽宿主范围。噬菌体分离物代表 65 种裂解谱；对 94 个噬菌体分离物的基因组大小分析允许将噬菌体分离物分类为 11 个组，随后的限制性片段长度多态性分析显示给定组内存在相当大的变异。我们的数据不仅表明奶牛场中存在大量多样化的沙门氏菌噬菌体分离物，而且还表明经常获得裂解最常见的引起牛沙门氏菌病的血清型的噬菌体分离物，这表明噬菌体可能在奶牛场沙门氏菌的生态学中发挥重要作用。
