Faculty of Medicine and Biomedical Sciences, University of Yaounde I, P,O, BOX 1364, Yaounde, Cameroon.
BMC Complement Altern Med. 2013 Sep 10;13:223. doi: 10.1186/1472-6882-13-223.
The use of edible plants is an integral part of dietary behavior in the West region of Cameroon. Dorstenia psilurus (Moraceae) is widely used as spice and as medicinal plant for the treatment of several diseases in Cameroon. The aim of this study is to investigate the cytotoxic and apoptotic potential of methanol extract of D. psilurus in human promyelocytic leukemia (HL-60) cells and prostate cancer (PC-3) cells.
Cytotoxicity of D. psilurus extract was tested in HL-60 and PC-3 cells using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay and flow cytometric methods
The methanol extract of D. psilurus have significant in vitro cytotoxic activity in HL-60 cells and PC-3 cells with IC₅₀ value of 12 ± 1.54 μg/ml and 18 ± 0.45 μg/ml respectively after 48 h. The mechanism of antiproliferative activity showed that after 24 h, D. psilurus extract induces apoptosis on HL-60 cells by the generation of reactive oxygen species (ROS) along with concurrent loss of mitochondrial membrane potential, modification in the DNA distribution and enhance of G2/M phase cell cycle.
The extract induces apoptosis of HL-60 cells associated with ROS production, loss of mitochondrial membrane potential and apoptotic DNA fragmentation.
食用植物是喀麦隆西部地区饮食行为的一个组成部分。多蕊蛇菰（桑科）在喀麦隆被广泛用作香料和药用植物，用于治疗多种疾病。本研究旨在研究多蕊蛇菰甲醇提取物在人早幼粒细胞白血病（HL-60）细胞和前列腺癌细胞（PC-3）中的细胞毒性和细胞凋亡潜力。
采用 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）还原法和流式细胞术检测多蕊蛇菰提取物对 HL-60 和 PC-3 细胞的细胞毒性。
多蕊蛇菰甲醇提取物对 HL-60 细胞和 PC-3 细胞具有显著的体外细胞毒性活性，48 h 后 IC₅₀ 值分别为 12 ± 1.54 μg/ml 和 18 ± 0.45 μg/ml。增殖抑制活性的机制表明，多蕊蛇菰提取物在 24 h 后通过产生活性氧（ROS），同时伴随着线粒体膜电位丧失、DNA 分布改变和 G2/M 期细胞周期增强，诱导 HL-60 细胞凋亡。
该提取物通过产生 ROS、线粒体膜电位丧失和凋亡 DNA 片段化诱导 HL-60 细胞凋亡。
