Meng Jie, Xiao Bo, Zhang Yu, Liu Jian, Xue Huadan, Lei Jing, Kong Hua, Huang Yuguang, Jin Zhengyu, Gu Ning, Xu Haiyan
1] Department of Biomedical Engineering, Institute of Basic Medical Sciences, Chinese Academy of Medical Science & Peking Union Medical College [2].
Sci Rep. 2013;3:2655. doi: 10.1038/srep02655.
A novel nanofibrous composite scaffold composed of super-paramagnetic γ-Fe2O3 nanoparticles (MNP), hydroxyapatite nanoparticles (nHA) and poly lactide acid (PLA) was prepared using electrospinning technique. The scaffold well responds extern static magnetic field with typical saturation magnetization value of 0.049 emu/g as well as possesses nanofibrous architecture. The scaffolds were implanted in white rabbit model of lumbar transverse defects. Permanent magnets are fixed in the rabbit cages to provide static magnetic field for the rabbits post surgery. Results show that MNP incorporated in the nanofibers endows the scaffolds super-paramagnetic responsive under the applied static magnetic field, which accelerates new bone tissue formation and remodeling in the rabbit defect. The scaffold also exhibits good compatibility of CK, Cr, ALT and ALP within normal limits in the serum within 110 days post implantation. In conclusion, the super-paramagnetic responding scaffold with applying of external magnetic field provides a novel strategy for scaffold-guided bone repair.
采用静电纺丝技术制备了一种由超顺磁性γ-Fe2O3纳米颗粒（MNP）、羟基磷灰石纳米颗粒（nHA）和聚乳酸（PLA）组成的新型纳米纤维复合支架。该支架对外部静磁场有良好响应，典型饱和磁化值为0.049 emu/g，且具有纳米纤维结构。将该支架植入白兔腰椎横突缺损模型中。术后在兔笼中固定永久磁铁，为兔子提供静磁场。结果表明，纳米纤维中掺入的MNP使支架在施加的静磁场下具有超顺磁响应，加速了兔缺损处新骨组织的形成和重塑。植入后110天内，该支架在血清中的CK、Cr、ALT和ALP也表现出良好的兼容性，均在正常范围内。总之，施加外部磁场的超顺磁响应支架为支架引导骨修复提供了一种新策略。
