Rabie G H, Aboul-Nasr M B, Al-Humiany A
Botany Department, Faculty of Science, Zagazig Univ., Zagazig, Sharkia Gov. 44519, Egypt.
Mycobiology. 2005 Mar;33(1):51-60. doi: 10.4489/MYCO.2005.33.1.051. Epub 2005 Mar 31.
Pot greenhouse experiments were carried out to attempt to increase the salinity tolerance of one of the most popular legume of the world; cowpea; by using dual inoculation of an Am fungus Glomus clarum and a nitrogen-fixer Azospirillum brasilense. The effect of these beneficial microbes, as single- or dual inoculation-treatments, was assessed in sterilized loamy sand soil at five NaCl levels (0.0~7.2 ds/m) in irrigating water. The results of this study revealed that percentage of mycorrhizal infection, plant height, dry weight, nodule number, protein content, nitrogenase and phosphatase activities, as well as nutrient elements N, P, K, Ca, Mg were significantly decreased by increasing salinity level in non-mycorrhized plants in absence of NFB. Plants inoculated with NFB showed higher nodule numbers, protein content, nitrogen concentration and nitrogenase activities than those of non-inoculated at all salinity levels. Mycorrhized plants exhibited better improvement in all measurements than that of non-mycorrhized ones at all salinity levels, especially, in the presence of NFB. The concentration of Na(+) was significantly accumulated in cowpea plants by rising salinity except in shoots of mycorrhizal plants which had K(+)/Na(+) ratios higher than other treatments. This study indicated that dual inoculation with Am fungi and N-fixer Azospirillum can support both needs for N and P, excess of NaCl and will be useful in terms of soil recovery in saline area.
进行了盆栽温室试验，试图通过双重接种一种丛枝菌根真菌（Glomus clarum）和一种固氮菌（巴西固氮螺菌）来提高世界上最受欢迎的豆类作物之一——豇豆的耐盐性。在灌溉水中含有五个NaCl水平（0.0~7.2 ds/m）的灭菌壤质砂土中，评估了这些有益微生物作为单一或双重接种处理的效果。本研究结果表明，在没有固氮菌的情况下，随着盐度水平的增加，未接种菌根的植物中菌根感染率、株高、干重、根瘤数、蛋白质含量、固氮酶和磷酸酶活性以及营养元素氮、磷、钾、钙、镁均显著降低。在所有盐度水平下，接种固氮菌的植物比未接种的植物表现出更高的根瘤数、蛋白质含量、氮浓度和固氮酶活性。在所有盐度水平下，接种菌根的植物在所有测量指标上均比未接种菌根的植物表现出更好的改善，尤其是在有固氮菌存在的情况下。除了接种菌根的植物地上部分钾/钠比高于其他处理外，随着盐度升高，豇豆植株中钠离子浓度显著积累。本研究表明，双重接种丛枝菌根真菌和固氮菌巴西固氮螺菌可以满足对氮和磷的需求，抵抗过量的NaCl，并且在盐碱地土壤恢复方面将是有用的。
