人体肥胖症中脂联素的合成和分泌与脂肪组织库和细胞大小的依赖性。
Adipose tissue depot and cell size dependency of adiponectin synthesis and secretion in human obesity.
机构信息
Center for Metabolic Research Veterans Affairs San Diego Health Care System; La Jolla, CA USA.
出版信息
Adipocyte. 2013 Oct 1;2(4):217-26. doi: 10.4161/adip.24953. Epub 2013 May 7.
Adiponectin is an insulin sensitizing fat cell (FC) hormone whose levels are related to adipose tissue (AT) mass and depot distribution. We hypothesized that the nature of AT expansion (hypertrophy vs. hyperplasia) contributes to obesity-related reductions in serum adiponectin and that this effect is influenced by the regional distribution of AT to subcutaneous (S) and visceral (V) depots. Thirteen obese subjects provided paired AT biopsies. Serum total and high molecular weight (HMW) adiponectin levels were determined by ELISA. Secretion was quantified following 24-h explant culture. FC size, number, % large, and % small FC were determined by microscopic analysis. Secretion of total adiponectin was highest by SAT (P = 0.008) and correlated more strongly with serum adiponectin (total: P = 0.015, r = 0.77; HMW: P = 0.005, r = 0.83) than did secretion by VAT (P = 0.05, r = 0.66 for both). FC size was greatest in SAT and correlated negatively with both serum (total: P = 0.01, r = -0.74; HMW: P = 0.03, r = -0.69) and secreted (total: P = 0.05, r = -0.72; HMW: P = 0.02, r = -0.87) adiponectin. The % small FC in SAT correlated positively with both serum (total: P = 0.006, r = 0.87; HMW: P = 0.009, r = 0.79) and secreted (total: P = 0.03, r = 0.75; HMW: P = 0.01, r = 0.92) adiponectin. VAT FC size correlated negatively with serum HMW adiponectin (P = 0.01, r = -0.76) but not with any measure of secretion. VAT had the greatest % small FC, which related positively to serum HMW (P = 0.004, r = 0.81) and to secreted total adiponectin (P = 0.02, r = 0.78). These studies indicate that differences in fat cell size and depot distribution of AT expansion are important influences on adiponectin in obesity.
脂联素是一种胰岛素敏感的脂肪细胞（FC）激素，其水平与脂肪组织（AT）质量和脂肪库分布有关。我们假设 AT 扩张的性质（肥大与增生）导致肥胖相关的血清脂联素减少，并且这种影响受到 AT 向皮下（S）和内脏（V）脂肪库分布的影响。13 名肥胖受试者提供了配对的 AT 活检。通过 ELISA 测定血清总和高分子量（HMW）脂联素水平。通过 24 小时外植体培养定量分泌。通过显微镜分析确定 FC 大小、数量、大 FC%和小 FC%。SAT 的总脂联素分泌最高（P = 0.008），与血清脂联素的相关性更强（总：P = 0.015，r = 0.77；HMW：P = 0.005，r = 0.83），而 VAT 的分泌则更低（P = 0.05，r = 0.66）。SAT 中的 FC 大小最大，与血清（总：P = 0.01，r = -0.74；HMW：P = 0.03，r = -0.69）和分泌（总：P = 0.05，r = -0.72；HMW：P = 0.02，r = -0.87）的脂联素均呈负相关。SAT 中的小 FC%与血清（总：P = 0.006，r = 0.87；HMW：P = 0.009，r = 0.79）和分泌（总：P = 0.03，r = 0.75；HMW：P = 0.01，r = 0.92）的脂联素呈正相关。VAT 的 FC 大小与血清 HMW 脂联素呈负相关（P = 0.01，r = -0.76），但与任何分泌测量值均无相关性。VAT 的小 FC%最大，与血清 HMW 脂联素呈正相关（P = 0.004，r = 0.81），与分泌的总脂联素呈正相关（P = 0.02，r = 0.78）。这些研究表明，脂肪细胞大小和 AT 扩张的脂肪库分布的差异是肥胖中脂联素的重要影响因素。
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