Opt Lett. 2013 Oct 1;38(19):3882-5. doi: 10.1364/OL.38.003882.
We propose a cross-correlation-based method to measure blood-flow velocity by using photoacoustic microscopy. Unlike in previous autocorrelation-based methods, the measured flow velocity here is independent of particle size. Thus an absolute flow velocity can be obtained without calibration. We first measured the flow velocity ex vivo, using defibrinated bovine blood. Then flow velocities in vessels with different structures in a mouse ear were quantified in vivo. We further measured the flow variation in the same vessel and at a vessel bifurcation. All the experimental results indicate that our method can be used to accurately quantify blood velocity in vivo.
我们提出了一种基于互相关的方法，利用光声显微镜来测量血流速度。与之前基于自相关的方法不同，这里测量的流速与颗粒大小无关。因此，无需校准即可获得绝对流速。我们首先使用去纤维牛血在离体条件下测量了血流速度。然后，在活体小鼠耳朵中不同结构的血管内定量了血流速度。我们进一步测量了同一血管和血管分叉处的血流变化。所有实验结果表明，我们的方法可用于准确地量化体内血流速度。
