小鼠杂交瘤抗体对DNA的特异性。III. 聚（dG）特异性单克隆抗体识别的核酸抗原决定簇。
Specificity of mouse hybridoma antibodies to DNA. III. Antigenic determinants of nucleic acids recognized by poly(dG) specific monoclonal antibodies.
作者信息
Koike T, Maruyama N, Tomioka H, Yoshida S
出版信息
Clin Exp Immunol. 1985 May;60(2):323-8.
Immunochemical properties of monoclonal autoantibodies to single stranded DNA (ssDNA), which were derived from systemic lupus erythematosus (SLE) prone mice and were specific for poly(dG), were studied using a hapten inhibition assay with oligo- and mononucleotides. Deoxyguanosine monophosphate (dGMP) completely inhibited the ssDNA binding of poly(dG) specific monoclonal antibodies. The affinity to deoxyguanosine was approximately 70% of dGMP, and to guanosine monophosphate (GMP) and to guanosine was 38% and 24%, respectively. In contrast, other purine monomers such as deoxyadenosine monophosphate (dAMP), adenosine monophosphate (AMP) and adenosine exhibited virtually no inhibitory effect on the ssDNA binding of these monoclonal hybridoma autoantibodies. By the same competitive inhibition enzyme immunoassay using deoxyguanosine oligonucleotides of various chain lengths, the minimal antigenic size of ssDNA encompassed by the combining site of the Fab portion of anti-ssDNA monoclonal autoantibodies was determined to be the tetra- or pentanucleotide.
对源自易患系统性红斑狼疮（SLE）小鼠且对聚（dG）具有特异性的单链DNA（ssDNA）单克隆自身抗体的免疫化学特性，采用寡核苷酸和单核苷酸的半抗原抑制试验进行了研究。脱氧鸟苷单磷酸（dGMP）完全抑制了聚（dG）特异性单克隆抗体与ssDNA的结合。对脱氧鸟苷的亲和力约为dGMP的70%，对鸟苷单磷酸（GMP）和鸟苷的亲和力分别为38%和24%。相比之下，其他嘌呤单体，如脱氧腺苷单磷酸（dAMP）、腺苷单磷酸（AMP）和腺苷，对这些单克隆杂交瘤自身抗体与ssDNA的结合几乎没有抑制作用。通过使用不同链长的脱氧鸟苷寡核苷酸进行相同的竞争性抑制酶免疫测定，确定抗ssDNA单克隆自身抗体Fab部分结合位点所包含的ssDNA的最小抗原大小为四核苷酸或五核苷酸。
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