Biskup Izabela, Golonka Iwona, Gamian Andrzej, Sroka Zbigniew
Department of Pharmacognosy, Silesian Piasts Wrocław Medical University, Wrocław, Poland.
Postepy Hig Med Dosw (Online). 2013 Sep 10;67:958-63. doi: 10.5604/17322693.1066062.
Phenols are the most abundant compounds in nature. They are strong antioxidants. Too high level of free radicals leads to cell and tissue damage, which may cause asthma, Alzheimer disease, cancers, etc. Taking phenolics with the diet as supplements or natural medicines is important for homeostasis of the organism.
The ten most popular water soluble phenols were chosen for the experiment to investigate their antioxidant properties using ABTS radical scavenging capacity assay and ferric reducing antioxidant potential (FRAP) assay.
Antioxidant properties of selected phenols in the ABTS test expressed as IC50 ranged from 4.332 μM to 852.713 μM (for gallic acid and 4- hydroxyphenylacetic acid respectively). Antioxidant properties in the FRAP test are expressed as μmol Fe2+/ml. All examined phenols reduced ferric ions at concentration 1.00 x 10-3 mg/ml. Both methods are very useful for determination of antioxidant capacity of water soluble phenols.
酚类是自然界中最丰富的化合物。它们是强大的抗氧化剂。自由基水平过高会导致细胞和组织损伤，这可能引发哮喘、阿尔茨海默病、癌症等。通过饮食摄入酚类作为补充剂或天然药物对机体的内稳态很重要。
选择十种最常见的水溶性酚类进行实验，使用ABTS自由基清除能力测定法和铁还原抗氧化能力（FRAP）测定法研究它们的抗氧化特性。
在ABTS测试中，所选酚类的抗氧化特性以IC50表示，范围为4.332 μM至852.713 μM（分别对应没食子酸和4-羟基苯乙酸）。FRAP测试中的抗氧化特性以μmol Fe2+/ml表示。所有检测的酚类在浓度为1.00×10-3 mg/ml时都能还原三价铁离子。这两种方法对于测定水溶性酚类的抗氧化能力都非常有用。
