绿原酸可抑制肠道病毒 71 在体外的复制和活力。

Chlorogenic acid inhibits the replication and viability of enterovirus 71 in vitro.

机构信息

Department of Clinical Laboratory, The Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu, China.

出版信息

PLoS One. 2013 Sep 30;8(9):e76007. doi: 10.1371/journal.pone.0076007. eCollection 2013.

Enterovirus 71 (EV71) is an etiology for a number of diseases in humans. Traditional Chinese herbs have been reported to be effective for treating EV71 infection. However, there is no report about the antiviral effects of CHA against EV71. In this study, plaque reduction assay demonstrated that the inhibitory concentration 50% (IC50) of CHA on EV71 replication is 6.3 µg/ml. When both CHA (20 µg/ml) and EV71 were added, or added post-infection at different time points, CHA was able to effectively inhibit EV71 replication between 0 and 10 h. In addition, CHA inhibited EV71 2A transcription and translation in EV71-infected RD cells, but did not affect VP1, 3C, and 3D expression. Furthermore, CHA inhibited secretions of IL-6, TNF-α, IFN-γ and MCP-1 in EV71-infected RD cells. Altogether, these results revealed that CHA may have antiviral properties for treating EV71 infection.

肠道病毒 71 型（EV71）是引起人类多种疾病的病原体。据报道，中草药对 EV71 感染有治疗作用。然而，目前尚无关于 CHA 对 EV71 的抗病毒作用的报道。在本研究中，噬斑减少试验表明，CHA 对 EV71 复制的半数抑制浓度（IC50）为 6.3 µg/ml。当同时加入 CHA（20 µg/ml）和 EV71 或在不同时间点感染后加入时，CHA 能够在 0 到 10 小时内有效抑制 EV71 的复制。此外，CHA 抑制 EV71 感染的 RD 细胞中的 2A 转录和翻译，但不影响 VP1、3C 和 3D 的表达。此外，CHA 抑制 EV71 感染的 RD 细胞中 IL-6、TNF-α、IFN-γ 和 MCP-1 的分泌。总之，这些结果表明 CHA 可能具有治疗 EV71 感染的抗病毒特性。