Department of Pharmacology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
Iran J Basic Med Sci. 2013 Aug;16(8):896-900.
OBJECTIVE(S): Methylsulfonylmethane (MSM) is a sulfur-containing compound found in a wide range of human foods including fruits, vegetables, grains and beverages. In this study the effect of MSM pretreatment on acetaminophen induced liver damage was investigated.
Male Sprague Dawley rats were pretreated with 100 mg/kg MSM for one week. On day seven rats were received acetaminophen (850 mg/kg, intraperitoneal). Twenty-four hours later, blood samples were taken to determine serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Tissue samples of liver were also taken for the determination of the levels of malondialdehyde (MDA); total glutathione (GSH), superoxide dismutase (SOD), and myeloperoxidase (MPO) activity together with histopathological observations.
High dose of acetaminophen administration caused a significant decrease in the GSH level of the liver tissue, which was accompanied with a decrease in SOD activity and increases in tissue MDA level and MPO activity. Serum ALT, AST levels were also found elevated in the acetaminophen-treated group. Pretreatment with MSM for one week was significantly attenuated all of these biochemical indices.
Our findings suggest that MSM pretreatment could alleviate hepatic injury induced by acetaminophen intoxication, may be through its sulfur donating and antioxidant effects.
甲基磺酰甲烷（MSM）是一种含硫化合物，存在于各种人类食物中，包括水果、蔬菜、谷物和饮料。本研究探讨了 MSM 预处理对乙酰氨基酚诱导的肝损伤的影响。
雄性 Sprague Dawley 大鼠用 100mg/kg MSM 预处理一周。第 7 天，大鼠接受乙酰氨基酚（850mg/kg，腹腔内注射）。24 小时后，采集血样测定血清天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）。还采集肝组织样本，测定丙二醛（MDA）水平；总谷胱甘肽（GSH）、超氧化物歧化酶（SOD）和髓过氧化物酶（MPO）活性，并进行组织病理学观察。
高剂量乙酰氨基酚给药导致肝组织 GSH 水平显著下降，同时 SOD 活性下降，MDA 水平和 MPO 活性升高。乙酰氨基酚处理组的血清 ALT、AST 水平也升高。用 MSM 预处理一周可显著减轻所有这些生化指标。
我们的研究结果表明，MSM 预处理可减轻乙酰氨基酚中毒引起的肝损伤，可能通过其提供硫和抗氧化作用。
