Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, 510275, China.
World J Microbiol Biotechnol. 2014 Mar;30(3):1085-92. doi: 10.1007/s11274-013-1528-5. Epub 2013 Oct 18.
To take full advantage of biochar as a soil amendment, the objective of this study was to investigate the effects of biochar addition on soil bacterial and fungal diversity and community composition. Incubation experiments with a forest soil (a red oxidized loam soil) with and without biochar amendment were conducted for 96 days. The culture-independent molecular method was utilized to analyze soil bacterial and fungal species after the incubation experiments. Results showed that bacteria and fungi responded differently to the biochar addition during the short-term soil incubation. Twenty four and 18 bacterial genara were observed in the biochar amended and unamended soils, respectively, whereas 11 and 8 fungal genera were observed in the biochar amended and unamended soils, respectively. Microbial taxa analysis indicated that the biochar amendment resulted in significant shifts in both bacterial and fungal taxa during the incubation period. The shift for bacteria occurred at the genus and phylum levels, while for fungi only at the genus level. Specific taxa, such as Actinobacteria of bacteria and Trichoderma and Paecilomyces of fungi, were enriched in the biochar amended soil. The results reveal a pronounced impact of biochar on soil microbial community composition and an enrichment of key bacterial and fungal taxa in the soil during the short time period.
为了充分利用生物炭作为土壤改良剂，本研究旨在研究生物炭添加对土壤细菌和真菌多样性及群落组成的影响。采用无生物炭和有生物炭添加的森林土壤（红色氧化壤土）进行了 96 天的培养实验。利用非培养分子方法分析了培养实验后的土壤细菌和真菌种类。结果表明，在短期土壤培养过程中，细菌和真菌对生物炭的添加有不同的反应。在添加和未添加生物炭的土壤中分别观察到 24 个和 18 个细菌属，而在添加和未添加生物炭的土壤中分别观察到 11 个和 8 个真菌属。微生物分类分析表明，在培养期间，生物炭的添加导致细菌和真菌的分类发生了显著变化。细菌的变化发生在属和门水平，而真菌仅在属水平上发生变化。特定的分类群，如细菌中的放线菌和真菌中的木霉属和拟青霉属，在添加生物炭的土壤中得到了富集。结果表明，生物炭对土壤微生物群落组成有明显的影响，并在短时间内富集了关键的细菌和真菌分类群。
