Emergency Unit, Hospital Clínico San Carlos.
J Sports Sci Med. 2011 Mar 1;10(1):45-51. eCollection 2011.
The purpose of the study was to determine if an intensive pre- season training program modifies the inflammatory status in professional soccer players and if this inflammatory profile may be associated with the physical state. We compared plasma protein biomarkers, using proteomics, and the physiological state and cardiac function in 12 professional soccer players and 9 recreational soccer players. Reduced cardiac low frequency [LF] after the pre- season training program previous competition with respect to recreational soccer players was found. No differences were found in cardiac high frequency, cardiac high frequency/low frequency ratio, tension index and oxygen volume consumption. Alpha-1-antitrypsin isotype-3, fibrinogen-gamma isotypes-1, 2 and 3 and vitamin-D-binding protein isotype-1 were reduced in professionals players compared with those in recreational players. However, an increased content of alpha-1-antitrypsin isotype-6 and alpha-1-antichymotrypsin 1 and 4 were found in professional soccer players. Spearman's analysis showed a positive correlation between LF and fibrinogen-gamma chain isotype 3; but LF was negatively correlated with alpha-antichymotrypsin isotype 4. Professional soccer players submitted to an intensive training showed differences in the content of plasma proteins associated with inflammatory/oxidative stress and thrombosis with respect to recreational soccer players. Proteomics analysis in combination with the analysis of cardiac function assessment may be useful to know more in depth molecular processes associated with sport and intensive exercise. Key pointsProteomics allow us to find differences in the plasma protein content in sportsmen.Just after pre-season training program, professional soccer players showed lower content of circulating proteins associated with inflammation compared to recreational soccer players.Proteomic analysis in combination with the analysis of cardiac function may be useful to know more in depth molecular inflammatory and oxidative processes associated with the sport.
本研究旨在确定强化赛季前训练方案是否会改变职业足球运动员的炎症状态，以及这种炎症特征是否与身体状况相关。我们比较了 12 名职业足球运动员和 9 名休闲足球运动员的血浆蛋白生物标志物（使用蛋白质组学）和生理状态及心脏功能。与休闲足球运动员相比，职业足球运动员在赛季前训练方案后的低频心脏（LF）降低。在高频心脏、高频/低频比值、张力指数和耗氧量方面没有差异。与休闲运动员相比，职业运动员的α-1-抗胰蛋白酶同工型-3、纤维蛋白原-γ 同工型-1、2 和 3 以及维生素 D 结合蛋白同工型-1 减少。然而，职业足球运动员中发现了 α-1-抗胰蛋白酶同工型-6 和 α-1-抗糜蛋白酶 1 和 4 的含量增加。Spearman 分析显示 LF 与纤维蛋白原-γ 链同工型 3 呈正相关；但 LF 与 α-抗糜蛋白酶同工型 4 呈负相关。与休闲足球运动员相比，接受强化训练的职业足球运动员在与炎症/氧化应激和血栓形成相关的血浆蛋白含量上存在差异。蛋白质组学分析结合心脏功能评估分析可能有助于更深入地了解与运动和剧烈运动相关的分子过程。关键点蛋白质组学使我们能够发现运动员血浆蛋白含量的差异。仅在赛季前训练方案后，职业足球运动员表现出与炎症相关的循环蛋白含量低于休闲足球运动员。蛋白质组学分析结合心脏功能分析可能有助于更深入地了解与运动相关的炎症和氧化过程的分子机制。
