Righolt Christiaan H, Slotman Johan A, Young Ian T, Mai Sabine, van Vliet Lucas J, Stallinga Sjoerd
Opt Express. 2013 Oct 21;21(21):24431-51. doi: 10.1364/OE.21.024431.
Various aspects of image filtering affect the final image quality in Structured Illumination Microscopy, in particular the regularization parameter and type of regularization function, the relative height of the side bands, and the shape of the apodization function. We propose an apodization filter without adjustable parameters based on the application of the Lukosz bound in order to guarantee a non-negative point spread function. Simulations of digital resolution charts and experimental data of chromatin structures and of actin filaments show artefact free reconstructions for a wide range of filter parameters. In general, a trade-off is observed between sharpness and noise suppression.
图像滤波的各个方面会影响结构照明显微镜中的最终图像质量，特别是正则化参数和正则化函数的类型、边带的相对高度以及变迹函数的形状。我们基于卢科斯界的应用提出了一种无可调参数的变迹滤波器，以保证点扩散函数非负。数字分辨率图的模拟以及染色质结构和肌动蛋白丝的实验数据表明，在广泛的滤波器参数范围内都能实现无伪影重建。一般来说，在清晰度和噪声抑制之间存在权衡。
